Team:HokkaidoU Japan/Practices

Human Practices

University Festival

We hald a workshop in Hokkaido University Festival that was held from June 11 to June 14, 2015. This was our third year participating in University Festival.

Poster Session

We made posters on following topics.

  • What is DNA
  • How DNA is familiar to us
  • What is synthetic biology
  • What is iGEM, and what are our activities

Experiment

We conducted an experiment detecting a DNA extraction from banana. Our aim was to make participants feel more familiar to DNA.

Protocol
  1. Crush one third of banana with spoon to break its cell wall.
  2. Mix 30 mL of 10% saltine water and 2.5 mL of detergent. Saltine water takes away negative electric charge form nucleic acid, and detergent breaks cell membrane.
  3. Pour 1 and 2 into a beaker through coffee filter to filtrate them.
  4. After 5 minutes, pour 40 mL of 100% ethanol into the beaker slowly.
  5. You’ll see some white lump, which is exactly the DNA!

Questionnaires

We took questionnaires to our participants, and we got 91 answers.

Results are shown on below.

Ratios

Impression about Gene Recombination

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