Team:Paris Saclay/Notebook/July/17

Contents

1 Friday 17th July
- 1.1 Lab Work

Friday 17th July

Lab Work

Ligation

by Pauline

In each tube:

3 µL Buffer DNA pol T4
1µL DNA pol T4
4µL H2O

BBa_J23101 (10µL) + BBa_S03518 (12µL)
BBa_S03518 (12µL) + BBa_B0015 (10µL)
BBa_B0015 (10µL) + BBa_I13602 (12µL)

Incubation 4h, 4°C

New culture

by Coralie

1696: Bad::Tet
1320:ClpP::Cm
1693:z1:Spect

On LB plates, incubation ON, 37°C

Transformation

by Johan

Transformation with ligation product:

BBa_J23101 + BBa_S03518 = BBa_K1707001
BBa_B0015 + BBa_I13602 = BBa_K1707002
BBa_S03518 + BBa_B0015 = BBa_K1707003

New culture on plates

by Pauline

BBa_J23101 + BBa_I13504
BBa_J23106 + BBa_I13504
BBa_J23117 + BBa_I13504
BBa_B0030
BBa_R0051

Plasmid extraction

by Pauline and Seong Koo

BBa_K1707000
BBa_K1399019
BBa_K1399005
BBa_K1399023

Cultures from the 16/15/2015 With the Nucleospin Kit from Macherey Nagel

Members present:

Instructors and advisors: Alice.
Students: Johan, Seong Koo, Coralie, Pauline

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