Team:Paris Saclay/Notebook/July/22
Contents
Wednesday 22nd July
Lab Work
Electrophoresis
by Johan and Pauline
- PCR Products:
- BBa_J23101
- BBa_K1707000
- Purification quantification:
- BBa_K1707002
- BBa_J23101
- BBa_R0051
- BBa_K098997
- BBa_K1707001
- Undigested plasmids:
- BBa_K1707001 #1 and #2
- BBa_K1707002 #1 and #2
- BBa_K1707003 #2
- BBa_J23101
- BBa_B0015
We can't see PCR products. We can suppose that they run out the gel.
Digestion
by Coralie
- Digestion by EcoRI
- Mix:
- 7µL EcoRI
- 7µL Buffer FastDigest 10x
- 42µL H2O
- Mix:
8µL in each tube + 2µL Plamid
- Biobricks:
- BBa_B0015
- BBa_J23101
- BBa_K1707003 #2
- BBa_K1707002 #1 and #2
- BBa_K1707001 #1 and #2
- Biobricks:
- Digestion by EcoRI + PstI
- Mix:
- 7µL EcoRI
- 7µL PstI
- 7µL Buffer FastDigest 10x
- 35µL H2O
- Mix:
8µL in each tube + 2µL Plamid
- Biobricks:
- BBa_B0015
- BBa_J23101
- BBa_K1707003 #2
- BBa_K1707002 #1 and #2
- BBa_K1707001 #1 and #2
- Biobricks:
Incubation 2h, 37°C
Electrophoresis
by Johan
We can conclude that K1707003 #2 isn't OK
Plasmid extraction
by Pauline and Seong Koo
Biobricks:
- BBa_K1707003 #4 to #9
- BBa_C0051
- BBa_E0240
- BBa_J06702
Soil experiment
by Audrey and Johan
Plates observation and count of CFU:
- Strain 1696:
- Dilution 1: more than 1000
- Dilution 0.1: more than 1000
- Dilution 0.01: 441 CFU
- Strain 1693:
- Dilution 1: more than 1000
- Dilution 0.1: more than 1000
- Dilution 0.01: 414 CFU
- Strain 1320:
- Dilution 1: more than 1000
- Dilution 0.1: 1744 CFU
- Dilution 0.01: 272 CFU
We take 1g of contaminated soil and treat it like yesterday to spread it on specific plates Incubation ON, 37°C
Electrophoresis of PCR products
Member present:
- Instructors: Alice
- Students: Johan, Seong Koo, Pauline, Audrey, Coralie
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.