Team:UCLA/Notebook/Protein Cages/27 July 2015
Today the new prefix primer (Cage Prefix #2) came in. We resuspended it, made more agarose gels, and ran a PCR with the first-round product containing the his-tag, the new prefix primer, and the suffix. We ran the PCR product on a gel, but got absolutely no bands at all. Tomorrow we will run another PCR with the new prefix primer and the first round suffix to see verify if the new prefix primer works. If it does, then the only problem is still the second suffix primer.
Tyler Lee --Wtleeiv 19:43, 27 July 2015 (CDT)