Team:KU Leuven/Wetlab
Research
We designed a circuit capable of forming patterns in a controlled way. Using a modified and temperature-sensitive lambda repressor (cI), we can trigger formation at desired points in time. This time-dependent controllability, together with the possibility to change many different parameters and output signals, leads to an enormous tunability in the creation of the patterns. Our mechanism will stimulate advancements in a variety of industrial processes like the creation of novel bio-materials. This fundamental project could also speed up medical research projects like tumor formation and tissue regeneration.
Background
A detailed description about the interaction between our two cells and the genetic circuit can be found here.
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Parts
When designing plasmids, we need to add tags and fluorescent proteins to quantify our parameters. We will also add extra restriction sites to easily generate biobricks
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Methods
Here you can find the following steps necessary to become our modified organism and detailed quantification methods to determine interesting parameters.
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Results
The results of our experiments will appear here.
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