Team:Paris Saclay/Notebook/July/27

Friday 27th July

Lab Work

New culture

by Seong Koo

Biobricks:

• BBa_K1707005
• BBa_K1707006
• BBa_K1707007

Ligation

by Pauline and Coralie

K1707008

• 2µL Tp 10x
• 1µL Ligase
• 11µL BBa_K1707003
• 6µL BBa_J23101

K1707010

• 1µL Tp 10x
• 1µL Ligase
• 12µL BBa_K1399005
• 10µL BBa_B0015
• 1µL H20

K1707009

• 2µL Tp 10x
• 2µL Ligase
• 10µL BBa_S03518
• 6µL BBa_R0051

K1707004

• 3µL Tp 10x
• 2µL Ligase
• 8µL BBa_K1707003
• 7µL BBa_R0051

Agarose gel 1%, 110V

We cut bands with a scalpel

Purification

by Audrey

Biobricks:

• BBa_K1707003
• BBa_K1399005
• BBa_B0015
• BBa_J23101

With PCR Clean-up/Gel extraction kit from Macherey-Nigel

Quantification

Biobricks:

• BBa_K1707003
• BBa_K1399005
• BBa_B0015
• BBa_J23101

Agarose gel 1% Migration 110V

Transformation

by Seong-Koo

Biobricks:

• BBa_K1707005
• BBa_K1707006
• BBa_K1707007

Soil experiment

by Seong Koo

Plates observation and count of CFU:

• Strain 1696:
  • Dilution 1: 424 CFU
  • Dilution 0.1: 75 CFU
  • Dilution 0.01: 1 CFU

• Strain 1693:
  • Dilution 1: 524 CFU
  • Dilution 0.1: 35 CFU
  • Dilution 0.01: 0 CFU

• Strain 1320:
  • Dilution 1: 111 CFU
  • Dilution 0.1: 13 CFU
  • Dilution 0.01: 0 CFU

We take 1g of contaminated soil and treat it like yesterday to spread it on specific plates Incubation ON, 37°C

Member present:

• Instructors: Alice
• Students: Coralie, Audrey, Pauline, Johan and Seong-Koo

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