Team:Glasgow/Notebook
We went to Dundee University to attend the Scotland IGEM team's meeting. We had so much fun in the meeting, sharing different ideas with each team.
The 4 strains of E. coli used are: MGI655ZI (which has tetR, and lacIq, DS941 (recF-), TOP10 (recA-), DH5α (recA-). We designed Phlf and SrpR promoters as BioBricks to be ordered from IDT as if their prefix and suffix were cut with EcoRI and SpeI.
We grew bacteria from a single colony from an agar plate in liquid culture to prepare for the dilution series. We designed the Phlf and SrpR respressor sequences with a prefix, B0032 ribosome binding site, TACTAG scar sequence, the repressor itself, TACTAGAG scar sequence, B0010 extended terminator with the help of Steve, and suffix.
We made TOP10 (which lacks methylation and restriction system, which makes it difficult to ‘cross’ with strains that have the systems) and DH5α (which lacks a restriction system, but has a modification system, ergo, DNA from it can be transferred to other cells) competent for transformation. Since we needed to have the cells in exponential growth phase, we pipetted 4 mL of overnight culture into 20 mL of fresh broth. We made chloramphenicol agar plates. We are also tested our dilution series on non-selective agar plates.