Team:UCLA/Notebook/Protein Cages/31 July 2015

Nithin's Notes

I ran a touchdown pcr today.

Conditions:

• 1-cycle-
  • 98 degrees- 30s
• 10-cycles
  • - 98 degrees- 10s
  • - 76-66 degrees (decrease by 1 degree each cycle)-30 seconds
  • - 72 degrees- 15s
• 15-cycles
  • - 98 degrees- 10s
  • - 66 degrees- 30s
  • - 72 degrees- 15s
• 1-cycle
  • - 72 degrees- 5 minute
  • - 4 degree hold.