Template:Team:Groningen/CONTENT/EXPERIMENTS/Grow a Biofilm on a Carrier Material

00:00, 25 March 2015 - 00:00, 26 March 2015

Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar. MSgg was prepared according to protocol. Carrier materials with LB agar as a medium:

Nylon positive charge (Boeringer, 1269299)

Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)

PVDF (Rocke, 03010040001)

Nitrocellulose 0.2 µm (invitrogen, LC2000)

Nylon 0.45 µm (Merck Milipore, HNWP04700)

Nitrocellulose 0.025 µm (Merck Milipore, VSWP02500)

Control (no carrier)

Carrier materials using MSgg agar as a medium

Nytran Plus 0.45 µm (Schleicher en Schuell, AH0495-1)

PVDF (Rocke, 03010040001)

Nitrocellulose 0.2µm (invitrogen, LC2000)

Nylon 0.45 µm (Merck Milipore, HNWP04700)

Nitrocellulose 0.025µm ( Merck Milipore, VSWP02500)

Control (no carrier)

Harm Ruesink

00:00, 25 March 2015 - 00:00, 26 March 2015

Plate 10 µL B. subtilis 3610 ComI overnight culture (OD: 2,28 (600nm) after 22h growth) on different carrier materials on LB agar and MSgg agar. MSgg prepared according to protocol.

Harm Ruesink