Team:Freiburg/Labjournals/cell free
![](https://static.igem.org/mediawiki/2015/8/85/Freiburg_wiki_background_2_parted_left.png)
![](https://static.igem.org/mediawiki/2015/f/f4/Freiburg_wiki_background_2_parted_right.png)
Labjournal Cell-free expression
![](https://static.igem.org/mediawiki/2015/8/85/Freiburg_wiki_background_2_parted_left.png)
![](https://static.igem.org/mediawiki/2015/f/f4/Freiburg_wiki_background_2_parted_right.png)
* pIG15_105 PCR products were extracted from gel and purified with Qiagen. The resulting DNA showed impurities around 230 nm in repeated measurements in the NanoDrop.
-> resulting DNA was not used for expression
HA-GFP-His6-His6 PCR product was retested in a control gel with a 20 µl sample. The resulting band hints at low DNA concentration * pIG15_105 PCRs from 28.07. and 29.07. were also retested by loading 20 µl in a gel. Since again no bands showed up, the PCRs should be repeated using smaller aliquots.