Template:Team:Groningen/CONTENT/EXPERIMENTS/Competent E. coli 2
00:00, 06 July 2015 - 00:00, 06 July 2015
A colony was picked from a plate (±14 days old, stored in fridge) and grown overnight in liquid media (5 mL LB in 50 mL tube) in shaker incubator at 37 °C, 200 rpm
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Tested competence of competent E. coli with Transformation efficiency kit (BBa_J04450, 50 pg/µL). Protocol: Heat Shock transformation (protocolbook).
1µL of 50pg/µL BBa_J04450
Plate on LB agar + CM:
100% (100µL)
Grow overnight in incubator at 37°C.