Team:Paris Saclay/Notebook/August/3

Monday 3rd August

Lab Work

New culture

by Pauline

Biobricks:

• BBa_K1707008
• BBa_I13600 (2014)

6 clones of each in 5mL LB + 20µg/mL of chloramphenicol

Incubation at 37°C, 250 rpm

Plasmid extraction

by Seong-Koo

Biobricks:

• BBa_K1707011 #1 to #6
• BBa_K1707021 #1 to #6
• BBa_K1707004 #1 to #6

Plasmid pIJ773

With the Macherey-Nagel Extraction kit

Soil experiment

by Coralie

Soil

Observation of plates (31/07/2015):

• Soil + H2O on new MCK: lots of colonies are visible
• Soil + LB on new MCK: lots of colonies are visible
• LB on new MCK: nothing
• MCK alone: nothing

We can suppose two different hypothesis:

• The soil is contaminated
• the MCK isn't OK for our experiment: now we add lactose in the preparation. We will try with sorbitol instead of lactose.

We try soil + H2O and soil +LB on:

• MCK + Sorbitol: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol
• MCK + Lactose: Without antibiotics, with Spectinomycine, Tetramycine or Chloramphenicol

Incubation ON, 37°C

Low Budget experiment

by Coralie

We wanted to participate to the Low Budget Challenge organized by Colombia. First step is to try to make an agar plate.

So we try two recipes:

• #1:
  • 0,27g stock cube
  • 0,675g agar agar
  • 0,135g baker's yeast

• #2
  • 0,36g stock cube
  • 0,675g agar agar

We boiled mix in glass cup of yoghurt (have been disinfected before use with alcohol) with micro-wave. We put 100µL of a GFP culture (BBa_J23101+BBa_I13504), spread by a wrench (have been disinfected before use with alcohol).

Incubation ON, 37°C

Member present:

• Instructors: Claire
• Students: Coralie, Audrey and Pauline

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