Template:Team:Groningen/CONTENT/EXPERIMENTS/DNA Isolation B. subtilis 168 and B. subtilis ComI
00:00, 06 May 2015 - 00:00, 07 May 2015
Streaked out B. subtilis 168 & B. subtilis ComI on LB agar from -80°C stock on LB agar plates and grown overnight at 37 °C.
00:00, 07 May 2015 - 00:00, 07 May 2015
Stored the plates in the fridge at 4 °C.
00:00, 12 May 2015 - 00:00, 12 May 2015
Picked up a colony from both 168 & ComI plate strain and put in LB medium (3 mL in culture tube). Grown overnight in shaker incubator (37 °C, 220 rpm).
00:00, 13 May 2015 - 00:00, 13 May 2015
DNA isolation (168 & ComI) with GenElute Bacterial Genomic DNA Kit.
Stored in -20 °C as:
168 DNA (50 µL, 21.9 ng/mL DNA)
ComI DNA (50 µL, 24.1 ng/mL DNA)