Team:Paris Saclay/Notebook/August/5

Wednesday 5th August

Lab Work

Quantification

by Pauline and Coralie

Biobricks:

• BBa_R0051
• BBa_E0022 (15ng/mL)
• BBa_E0422 (20ng/mL)
• BBa_K1707000 (20ng/mL)
• BBa_K1707004 (15ng/mL)
• BBa_K1707011
• BBa_K1707012
• BBa_K1707013 (20ng/mL)
• BBa_K1707019 (15ng/mL)

Agarose gel, 1% Migration 90V

Ligation

by Pauline

Biobricks: BBa_K1707020 : BBa_K1707004 + BBa_K1707019

Mix:

• 6,5 µL BBa_K1707019
• 9,5 µL BBa_K1707004
• 2 µL Buffer 10x
• 1 µL Ligase
• 1 µL H2O

BBa Test : BBa_K1707004 + BBa_K1707013

Mix:

• 5 µL BBa_K1707013
• 7,5 µL BBa_K1707004
• 1,5 µL Buffer 10x
• 1 µL Ligase

BBa_K1707027 : BBa_K1707000 + BBa_E0022

Mix:

• 5 µL BBa_K1707000
• 9 µL BBa_E0022
• 1,5 µL Buffer 10x
• 1 µL Ligase
• 3,5 µL H2O

BBa_K1707028 : BBa_R0051 + BBa_E0422

Mix:

• 6,25 µL BBa_R0051
• 5,5 µL BBa_E0422
• 1,5 µL Buffer 10x
• 1 µL Ligase
• 0,75 µL H2O

Rehydratation

by Audrey

BBa_R0040

Transformation

by Audrey and Coralie

• BBa_R0040
• BBa Test
• BBa_K1707028
• BBa_K1707027
• BBa_K1707020

Digestion

by Pauline

XbaI + PstI:

• BBa_K1707012
• BBa_K1707011
• BBa_I13600

SpeI + Pst:

• BBa_R0051
• BBa_K1707008

EcoRI + SpeI:

• BBa_K1707004

XbaI + EcoRI:

• BBa_I13602

Mix:

• 10 µL Plasmide
• 1 µL Enzyme 1
• 1 µL Enzyme 2
• 2 µL Buffer FastDigest 10x
• 6 µL H2O

Soil experiment

by Coralie

Water

Observation of plates (04/08/2015) We can see a lots of pink colony. Sea water + 1696 (Dil:1): We can see that the MCK is all yellow.

J1: we take 100µL of each water and put it on plates with specific antibiotic:

• MacConkey + Spectinomycin
• MacConkey + Tetracyclin
• MacConkey + Chloramphenicol

And only MacConkey for the Water + LB

Incubation ON, 37°C

Low Budget Challenge

by Coralie

We can't say anything about the agar: is it a bacterial mat or nothing is on the plate? We choose to incubate another time over night plates at 37°C

Member present:

• Instructors: Claire
• Students: Coralie, Audrey and Pauline

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