Team:Aalto-Helsinki/LabBook

• Project
• Modeling
  • Models of propane pathway
    • Kinetics of propane pathway
    • Model of CAR activation
  • Synergy model
  • Micelle model
  • About cellulose pathway
• Laboratory
  • Lab Book
  • Protocols
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  • Continuous production
  • Submitted parts
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• Practices
  • Combining modeling and experimentation in iGEM
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  • Collaboration Platform
  • Collab seeker
  • Interlab study
    • InterLabBook
• People
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• 
• 

• Week 1

• Week 2

• Week 3

• Week 4

• Week 5

• Week 6

• Week 7

• Week 8

• Week 9

• Week 10

• Week 11

• Week 12

• Week 13

• Week 14

• Week 15

• Week 16

• Week 17

• Week 18

Lab Book

An example of what the table could look like:

Construct worked with	Things done						Lab 1.1.2015 Monday
CAR	Here is my text	Here is my text	Here is my text	Here is my text	Here is my text	Here is my text	Here is my text, there is lot of it
another CAR?	Here is my text	Here is my text	Here is my text	Here is my text	Here is my text	Here is my text	Here is my text, there is lot of it
Ado?	Here is my text	Here is my text	Here is my text	Here is my text	Here is my text
Ado cont.			Here is my text	Here is my text	Here is my text	Here is my text	Here is my text, there is lot of it

Week 1

Construct worked with	Things done						May 18th - May 22nd
Plates	Making Cm plates	Stored in +4°C
Competent Cells	Preparing solutions for competent cells	Stored in +4°C
Competent Cells	o/n culture of E. coli TOP10	Chemical competent cells protocol	Tranformation efficiency kit
Competent Cells	o/n culture of E. coli BL21(DE3)	Chemical competent cells protocol	Tranformation efficiency kit	Colonies are the wrong color
Propagations	Transformed   AH001-AH008 into TOP10	AH001-AH004 & AH006	Stored in +4°C
		No colonies for    AH005, AH007 or AH008

Week 2

Construct worked with	Things done						May 25th - May 29th
Propagations	Transformed AH007-AH009, AH005 & AH019 into TOP10	AH007-AH009 & AH019	o/n cultures	MiniPrep
		No colonies for AH005	Re-transform into TOP10	Plates in rt over weekend
Competent Cells	o/n culture of BL21(DE3) transformants	MiniPrep	PCR with VF2 & VR	Smeared gels
Assembly Testing RBS's	Restrict AH001-AH004 & AH006-AH008	Ligation	AH002+   AH004+AH008 -> AH014	Repeat ligation	Restrict & extract from gel	Transformation into TOP10	Plates in rt over weekend
			AH001+    AH006+AH008 -> AH011	Repeat ligation	Restrict & extract from gel	Transformation into TOP10	Plates in rt over weekend
			AH001+    AH003+AH008 -> AH013	Repeat ligation	Restrict & extract from gel	Transformation into TOP10	Plates in rt over weekend
Propagations	o/n culture of   AH001-AH004 & AH006	MiniPrep

Week 3

Construct worked with	Things done						June 1st - June 5th
Assembly Testing RBS's	No colonies for AH011 or AH014	re-assemble	Restrict & extract from gel	Ligate & tranform into TOP10	No colonies
Assembly Testing RBS's	AH005 & AH013 OK	o/n cultures	MiniPrep
Propagations	Transform     AH020 & AH021 into TOP10	Plates in rt  over weekend

Week 4

Construct worked with	Things done						June 8th - June 12th
Assembly Propagations	o/n cultures for AH020 & AH021	MiniPrep
Assembly Testing RBS's	Re-assembling    AH011, AH013 & AH014	Transform into TOP10	o/n cultures	MinPrep
Assembly Testing RBS's	Assembling AH015	Ligate AH002+   AH005+AH008	Restrict & extract from gel	Transform into TOP10	o/n cultures	MiniPrep
Propagations	Transform     AH020 & AH021 into TOP10	Plates in rt over weekend

Week 5

Week 6

Construct worked with	Things done						Week 6
Assembly Testing RBS's	Re-assembling AH016 & AH018	Transformation into BL21(DE3)	No colonies
Testing RBS's	o/n cultures of   AH016 & AH018 in TOP10	MiniPrep	Transform into BL21(DE3)	Plates in rt over the weekend
Measurement Testing RBS's	AH018 in BL21(DE3)	o/n culture	IPTG induction &     blue chromoprotein measurement	No blue chromoprotein
		Refresh on IPTG plate	No blue chromoprotein
Producing linearized backbones	o/n cultures of AH007-AH009	MiniPrep	Restrict & extract from gel

Week 7

Construct worked with	Things done						Week 6
Preparations Testing RBS's	Transform AH007-AH009 into TOP10	No colonies (mistake in protocol)	Use iGEM's linearized backbones
Testing RBS's	o/n cultures of   AH016&AH018 in TOP10	IPTG induction	No Blue chromoprotein
Measurement Testing RBS's	Refresh AH016 & AH018 on IPTG plate	No blue chromoprotein
Violacein construct	PCR AH20 with P012&P013	PCR purification	VioA waits for 2nd PCR
	PCR AH20 with P024&P025	PCR unsuccesful
	PCR AH20 with P035&P025	PCR purification	VioE waits for 2nd PCR

Week 8

Construct worked with	Things done						Lab 1.1.2015 Monday
Constructing Cellulose system	PCR AH021 with P019&P020	Problem with PCR machine	Re-do PCR	PCR purifcation	BglX waits for 2nd PCR
	AH029 into TOP10	Transformants stored in +4°C
Preparations	Preparing   PK BL21(DE3) competent cells	Cells stored in -80°C
Testing RBS's	Transform AH016 & AH018 into PK BL21(DE3)	Refresh transformants on IPTG plates	No blue chromoprotein
Violacein Construct	Gradient PCR for AH020 with P024&P025	PCR purification

Week 9

Week 10

Week 11

Week 12

Week 13

Week 14

Week 15

Week 16

Week 17

Week 18