Team:TCU Taiwan/Project/Overview

Members Instructors Attributions Overview Antimicrobial Peptides Signal Peptide Medical Dressing Basic Part Composite Part Protein structure Modeling Overview Laboratory Practices Peptides production Disinfection efficiency Cell experiments Human Practices Collaborations Life Lab

AMP. coli

AMPs have an extensive ability in disinfect. Unlike antibiotics, AMPs can puncture the cell membrane to kill the bacteria therefore by passing bacterial antibiotic drug resistance mechanisms. Two kinds of AMPs were selected as our reagents: Epinecidin-1 and Signiferin.           Epinecidin-1 is a peptide comes from fish ( Epinephelus coioides ), and Signiferin is comes from tree frogs  ( Crinia signifera ). Both are extracted from the skin mucus. In addition, epinecidin-1 has the ability to help wounds healing and has been proven by animal studies. Moreover, signiferin have great ability in disinfect Methicillin-Resistant Staphylococcus aureus (S. aureus), and had already been kindly proved by the TU-Delft 2013 iGEM team. Combining these two properties, we believe that can alleviate the serious problem of skin injury.         To produce AMPs and control AMPs expression, we apply the Lac operon and ligate the DNA of signal peptide into E. coli to help AMPs secret into culture medium. Next, to prove that AMPs have the extensive ability in disinfection and helps the wound healing, selected cells and bacteria were tested in vitro, including the squamous epithelial cell and endothelial cell of the blood vessel and MRSA, and mice were used in vivo. Ultimately, create a wound dressing based on the above procedure.        An excellent dressing made of AMPs will make a fast recovery.

Antimicrobial peptide    • Epinecidin-1:     1. from the skin mucus of Epinephelus coioides a kind of fish.     2. has function of killing bacteria.     3. In addition, it has the ability to help wounds healing and has been proven by animal studies.    •  Signiferin:     1. from the skin mucus of Crinia signifera a kind of tree frog.     2. have function of killing bacteria.     3. have great ability in disinfect Methicillin-Resistant Staphylococcus aureus (MRSA).     4. had already been kindly proved by the 2013 TU-Delft iGEM team.     Signal peptide:     1. helps AMPs to secret out of E. coli.     2. from Streptomyces lividans to trasport chitinase C to secretion system, which has been proven to work in E.coli         by reference.     Wound dressing:        Based on AMPs to develop into a potential material of wound dressing.

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