Team:UCLA/Notebook/Spider Silk Genetics/12 August 2015

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08/12/2015

Colony PCR for M1 SwqAB 1 & 2 and M1-9(T7)

2X Taq MM	6.25 uL
VF Primer	0.625 uL
VR Primer	0.625 uL
Template	1 uL
ddH2O	4 uL

95C	3 min
95C	30 sec
72C	25 sec (M1SeqAB)/ 1min 40 sec (M1-9)
	x30
72C	5 min
12C	Hold