Team:UCLA/Notebook/Spider Silk Genetics/11 August 2015

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08/11/2015

BsaI Digestion of M2AB, BC and CA

	2AB	2BC	2CA
Cutsmart	5 uL	5 uL	5 uL
Template	35 uL	19.5 uL	18.2 uL
ddH2O	6 uL	21.5 uL	22.8 uL
BsaI	4 uL	4 uL	4 uL

50 C for 2hr 65 C for 20 min