Team:UCLA/Notebook/Spider Silk Genetics/11 August 2015

iGEM UCLA




08/11/2015

BsaI Digestion of M2AB, BC and CA

2AB 2BC 2CA
Cutsmart 5 uL 5 uL 5 uL
Template 35 uL 19.5 uL 18.2 uL
ddH2O 6 uL 21.5 uL 22.8 uL
BsaI 4 uL 4 uL 4 uL

50 C for 2hr 65 C for 20 min

ICA M1-15

Results

BsaIDig0811.jpg