Team:Technion Israel/Lab Notebook/Overview
Lab Notebook Overview
Week 1
Bacillus
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E.coli
Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.
Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.
Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.
Active team members this week: Adi and Alexey
Modeling
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Comb
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NADPH
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Week 2
Bacillus
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E.coli
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Modeling
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Comb
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NADPH
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