Team:Technion Israel/Lab Notebook/Overview
Lab Notebook Overview
Week 1: May 2-8
Bacillus
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E.coli
Planned the Gibson reaction for our gBlock of 3α-HSD and the plasmid N155 (from our mentors) containing pT7, RBS and mcherry.
Ordered the gBlock of 3α-HSD, which was optimized for E. coli codon usage. We also ordered primers for the Gibson reaction for the gBlock.
Performed reverse PCR to the N155 plasmid and carried out a DPN1 reaction on the product.
Active team members this week: Adi and Alexey
Modeling
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Comb
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NADPH
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Week 2: May 9-15
Bacillus
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E.coli
Our gBlock arrived!!!
We performed PCR on the 3αHSD gBlock with only 10 cycles (as recommended), but received no band in the gel
We performed PCR on the 3αHSD gBlock in higher temperatures, but still received no bands in the gel
Active team members this week: Adi
Modeling
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Comb
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NADPH
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Week 3: May 16-22
Bacillus
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E.coli
Performed PCR on the 3αHSD gBlock in lower temperatures, no bands in the gel.
Active team members this week: Alexey
Modeling
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Comb
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NADPH
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