Team:UiOslo Norway/Experiments/PCR taq

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PCR with Taq DNA Polymerase (NEB):

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1. Reaction Setup:
   
   2.5 µl 10X Standard Taq Reaction buffer
   2.5 µl dNTPS [2 mM]
   0.5 µl Forward Primer [10 µM]
   0.5 µl Reverse Primer [10 µM]
   1 µl Template DNA
   0.125 µl Taq DNA Polymerase
   17.875 µl MilliQ Water
   

   ---

   25 µl Total Reaction




2. PCR Program:
   

   First Name	Last Name	Points
   Jill	Smith	50
   Eve	Jackson	94
   John	Doe	80