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- <li><a href="#directedmutagenesispcr">Directed mutagenesis PCR for restriction site elimination using a plasmid template </a></li> <li><a href="#pcramplification1">PCR amplification (applied to lcp)</a></li>24 KB (2,967 words) - 23:52, 20 November 2015
- <li>1μl dye to 5μl DNA is sufficient for PCR products and restriction digests.</li> <h4>PCR Components</h4>26 KB (2,428 words) - 03:18, 19 September 2015
- ...e combined <i>mamW+RFP+laccase</i> and <i>RFP</i>+<i>laccase</i> by fusion PCR (Fig. 1B).<br> ...amW</i> and <i>RFP</i> and <i>laccase</i> were amplified by PCR using high fidelity DNA polymerase. <strong>(B)</strong>36 KB (3,710 words) - 02:48, 19 September 2015
- ...style="width:500px; margin-top:-25px;"><h3>PCR Protocol using Phusion High-Fidelity DNA Polymerase</h3></div> <p><strong>PCR </strong><br />8 KB (1,052 words) - 05:45, 22 August 2015
- <li>Pursue the protocol by the NucleoSpin® Gel and PCR Clean-up protocol</li> <h4>3. PCR amplification</h4>17 KB (2,170 words) - 23:29, 18 September 2015
- ...CGCTACTAGTA) Primers. After that, PCR products were purified with QIAquick PCR Purification Kit. <br> <h3>PCR mixture </h3>5 KB (804 words) - 04:39, 16 September 2015
- ...nealing and extension, simulating <i>in vivo</i> DNA replication progress. PCR has radically altered molecular biology; via this technique, a trace amount ...ously. All these methods need a thermal cycler; Nested PCR and Multiplexed PCR need more than two pairs of primers, which may lead to the generation of pr39 KB (4,417 words) - 14:54, 17 November 2015
- <p class="text"><a href="#2206c">PCR Set-up</a></p> ...text"><a href="#2206d">Setting up Agarose Gel for Electrophoresis of 22/06 PCR Products</a></p>14 KB (1,920 words) - 15:44, 6 July 2015
- ...of PoxB, Pta, sdhA and some other enzymes could be knocked out to ensure a high succinic acid yield <sup>[15]</sup>. <!-- Error-Prone PCR -->43 KB (6,183 words) - 20:20, 18 September 2015
- <li>PCR'd off template</li> <p>PCR Reaction:</p>12 KB (1,619 words) - 23:38, 20 May 2015
- ...ks LuxI-His, RBS-LuxI-His and CheZ-GFP, RBS-CheZ-GFP by high fidelity tail-PCR, digestion and ligation in pSB1C3<br/> ...>, testing colonies by PCR and preparing for sequencing. The high fidelity PCR and cloning of other potential BioBricks was repeated. <br/>58 KB (8,236 words) - 10:16, 20 October 2015
- <h1 class="sectionedit1"><a name="pcr" id="pcr">PCR</a></h1> <!-- EDIT1 SECTION "PCR" [1-19] -->5 KB (690 words) - 07:05, 20 November 2015
- <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>25 KB (3,384 words) - 14:18, 17 September 2015
- <h6> 12. Add 50 uL PCR water to the center of the QIAprep spin column to elute DNA, let stand for <h2>PCR using Q5 High-Fidelity DNA Polymerase</h2>31 KB (4,008 words) - 12:01, 14 September 2015
- = PCR Amplification and Gel Extraction of InterLab Devices = == PCR Reaction & Thermocycler Setup ==14 KB (1,900 words) - 01:48, 16 August 2015
- <div class="textBox" id="PCR"> <h3>PCR protocol</h3>37 KB (5,389 words) - 10:32, 19 November 2015
- ='''Colony PCR of 5/4 Transformation'''= ====Colony PCR Reaction====11 KB (1,333 words) - 06:25, 10 July 2015
- <h4> 1) PCR Amplification using TaKaRa Ex Taq DNA polymerase</h4> <li> Set up the following cycles in a PCR machine42 KB (6,658 words) - 21:57, 19 November 2015
- <h3 class="sectionedit4">PCR</h3> ...CR approaches were used. In general, PCRs were performed with Phusion High Fidelity DNA Polymerase (NEB). The components of the reaction mixture were used in d19 KB (3,058 words) - 16:56, 28 August 2015
- ...="button" class="btn btn-info" data-toggle="collapse" data-target="#demo9">PCR protocol with EconoTaq PLUS 2X</button> ...fo" data-toggle="collapse" data-target="#demo10">PCR protocol with Q5 High Fidelity DNA Polymerase</button>31 KB (3,932 words) - 00:07, 19 September 2015