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- <span class="c2">HO-Poly-KanMX4-HO amplification (reverse) + tail</span> <span class="c2">gBlock2 amplification (forward) + tail</span>15 KB (1,977 words) - 15:31, 14 August 2015
- <p>This is host-specific subunit of the short tail fibre protein of bacteriophage lambda. The C-terminus of the protein (ORF-3 <h3><b>stf (short tail fibre) gene (BBa_K1846004)</b></h3>8 KB (1,199 words) - 21:29, 16 November 2015
- ...en color of inland area on the earth. Both chromoprotein followed with aav tail to obtain the color change distinctly from green to yellow. We introduced a ...the chromoprotein to make it more vulnerable to the attack from endogenous tail-specific proteases. Two tails were chosen for our testing, LVA tag and AAV72 KB (7,426 words) - 16:17, 4 October 2015
- <h3><b>tfa (tail fibre assembly) gene circuit (BBa_K1846001)</b></h3> <p>This part provides the gene sequence for tfa (tail fibre assembly) protein, together with a TetR repressible promoter (<a href11 KB (1,716 words) - 21:16, 16 November 2015
- ...d be translated into the protein. Second, the gRNA must be free from polyA tail and 5’ cap to avoid relocation into the cytoplasm and degradation</p> ...The other CGC1 part, the synthetic poly A tail, is used as the new poly-A tail after ribozyme cleavage ensuring the cas9 mRNA translocation and translatio8 KB (1,343 words) - 03:31, 19 September 2015
- ...w cows are much more accepting of a vet approaching and working with their tail as oppose to their necks.</p> ...tically Phil said this would not be possible. Collection of blood from the tail into a tube the size of an Eppendorf would not only be extremely difficult46 KB (7,231 words) - 03:08, 19 September 2015
- <tr><td>398</td><td>L</td><td>tail length</td><td>0.8</td><td>cm</td><td><a href="#atlas">[1]</a></td><td><a h <tr><td>399</td><td>l</td><td>max tail width</td><td>0.07</td><td>cm</td><td><a href="#atlas">[1]</a></td><td><a h265 KB (43,510 words) - 20:28, 18 September 2015
- ...eraction, the larger beta barrel is shown in blue and the small c-terminal tail show in red.</p> ...f OBP2A in exchange for our tighter binding synthetic tail. This synthetic tail could then be attached to a florescent nanoparticle and used in the detecti41 KB (6,123 words) - 12:47, 4 October 2015
- <br><span style="color:#9A1717"> - Homology tail on gene PHO85</span> <br><span style="color:#9A1717"> - Homology tail on gene PHO80</span>26 KB (4,138 words) - 14:04, 30 October 2015
- <h3>TFA (tail fibre assembly) gene circuit (BBa_K1846001)</h3> ...ts in the assembly of the stf (short tail fibre) protein into a functional tail fibre. This part provides the gene sequence for tfa, together with a TetR r2 KB (308 words) - 17:50, 28 August 2015
- ...le pork belly ball tip short ribs biltong meatball chuck. Pork chop ribeye tail short ribs, beef hamburger meatball kielbasa rump corned beef porchetta lan ...k ribeye landjaeger beef kevin tail meatball pastrami prosciutto pancetta. Tail kevin spare ribs ground round ham ham hock brisket shoulder. Corned beef tr9 KB (1,220 words) - 21:33, 1 September 2015
- ...w cows are much more accepting of a vet approaching and working with their tail as oppose to their necks. </p> ...tically Phil said this would not be possible. Collection of blood from the tail into a tube the size of an Eppendorf would not only be extremely difficult2 KB (404 words) - 13:16, 9 September 2015
- ...reating some BioBricks. First up, the most basic one: ORF314, (a piece of) tail fibre protein of lambda phage. After cloning the gene into iGEM standard ba ...by the end of the week we would have a complete bacteriophage lambda stf (tail fibre protein) gene with a His-tag for easy purification. But alas! In typi33 KB (4,872 words) - 21:27, 16 November 2015
- ...oBricks LuxI-His, RBS-LuxI-His and CheZ-GFP, RBS-CheZ-GFP by high fidelity tail-PCR, digestion and ligation in pSB1C3<br/> - Optimizing the tail PCR to make Biobricks <br/>58 KB (8,236 words) - 10:16, 20 October 2015
- <li> The polyA tail is a sequence of 50 adenines. This particular sequence aims to replace the <br>[4] [4] Preiss T, Muckenthaler M, Hentze MW. Poly(A)-tail-promoted translation in yeast: Implications for translational control. 199855 KB (8,059 words) - 23:46, 7 October 2015
- ...rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail which can vary in length (Han <i>et al</i>. 2014). The mono-rhamnolipid wil ...efficiency. The RhlA creates the fatty acid tail and the RhlB connects the tail to a rhamnose molecule. (Han <i>et al</i>. 2014) The genes that express the12 KB (1,820 words) - 21:58, 19 November 2015
- ...non-specificity in primer binding. While other techniques such as head-to-tail assembly or concatemerization have been developed to facilitate spider silk ...ic sequences, then assemble the monomers into the final construct. Head-to-tail cloning assembles gene constructs by ligating two plasmid halves together.20 KB (3,083 words) - 21:45, 18 September 2015
- ...wer, K., Kuperwasser, N., Merrikh, H., & Rosbash, M. (2004). A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript. <I> ...short DNA duplexes with at least a 10 nucleotide long 5’single-stranded tail for tight binding and efficient unwinding. [1]</p>54 KB (8,522 words) - 02:18, 19 September 2015
- ...pecificity is <a href="https://2015.igem.org/Team:Birkbeck/Composite_Part">tail fibre assembly (TFA) protein</a>.</p>4 KB (630 words) - 21:56, 18 September 2015
- ...li. Furthermore, the sequence shows great (>50%) homology with the gene 37 tail fibre protein of bacteriophage T4.</p>2 KB (295 words) - 12:04, 11 August 2015