Team:Czech Republic/Parts
Parts
Description
We relied heavily on two promoters from the Registry (pGAL1 and pCUP1), and so we extensively characterized them both and submitted the results to the Registry as a part of the Gold medal requirements.
Basic Parts
Several Basic parts were constructed during the summer. We contributed to the Registry with 8 different basic parts, ready to use in Saccharomyces cerevisiae. Function of all these parts was experimentally validated.
We sent 4 different mating pheromones:
- Mating pheromone (S. cerevisiae) - BBa_K1690000
- Mating pheromone (C. albicans) - BBa_K1690001
- Mating pheromone (C. tropicalis) - BBa_K1690002
- Mating pheromone (L. elongisporus) - BBa_K1690003
And with the sequences of 2 different pheromone receptors:
- Pheromone receptor (C. albicans) - BBa_K1690004
- Pheromone receptor (C. parapsilosis) - BBa_K1690005
The BioBricks above were constructed by Module 2, where you can find details of their construction and function.
Also, 3 different reporters ready to use in Saccharomyces were prepared and sent:
- pADH1_GFP (Yeast reporter) - BBa_K1690006
- pasCYC1v3_GFP (Yeast reporter) - BBa_K1690007
- pTv3_GFP (Yeast reporter) - BBa_K1690008
These BioBricks were constructed by Module 1, where you can find details of their construction and function.
Part Collection
Our submitted parts can be divided into two separate collections:
Signals & Receptors
- Mating pheromone (S. cerevisiae) - BBa_K1690000
- Mating pheromone (C. albicans) - BBa_K1690001
- Mating pheromone (C. tropicalis) - BBa_K1690002
- Mating pheromone (L. elongisporus) - BBa_K1690003
- Pheromone receptor (C. albicans) - BBa_K1690004
- Pheromone receptor (C. parapsilosis) - BBa_K1690005
Yeast reporters
- pADH1_GFP (Yeast reporter) - BBa_K1690006
- GFP under the control of constitutive pADH1 promoter
- pasCYC1v3_GFP (Yeast reporter) - BBa_K1690007
- GFP under the control of a MATa specific constitutive pasCYC1v3 promoter
- pTv3_GFP (Yeast reporter) - BBa_K1690008
- GFP under the control of repressible pTet promoter