Team:EPF Lausanne/Team/Timeline

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Timeline

  • The bible

    The E. Coli part of our project is strongly based on the paper of Bikard et al. "Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system". Bikard sent us his strains JEN202, JEN202+PWJ89 and DH5A+PWJ66. We also ordered a plasmid containing dCas9 and the synthetic DNA encoding fou our gRNAs.

  • Blast

    02.04.2015

    In our project dCas9 target DNA sequences using gRNAs and acts as an activator or an inhibitor depending on its position. Axel and Cyril created a C++ program to blast random generated gRNA sequences on the E. Coli or Yeast genome.

  • And the winner is...

    26.03.2015

    ...the new framework for biologic circuits using dCas9.

  • The lab starts!

    24.03.2015 - 26.03.2015

    TAs start to train us in the lab: medium and agar plates preparation

  • Brainstorming finalists

    19.03.2015

    Three cool ideas came out of the brainstorming:

    • A new framework for biologic circuits

    • Lung cancer therapy using dCas9

    • Cell-cell communication through exosomes

  • Brainstorming

    26.02.2015 - 12.03.2015

    The team is split into small groups, brainstorming for an interesting project that will occupy us during the summer.

  • First meeting: iGEM officially starts!

    19.02.2015

    First meeting with everyone: 12 teammates, 7 TAs and 3 advisors.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits