Team:EPF Lausanne/Attributions

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Attributions

Team attributions

All research was done by the students on our team.

Experiments were designed and conducted by Emilie Cuillery, Paola Malsot, Rocco Meli, Ari Sarfatis, Victor Steininger, Grégoire Thouvenin and Nina van Tiel in E. coli and by Joseph Heng, Vincent Jacquot, Cyril Pulver, Loïc Steiner and Axel Uran in S. cerevisiae. Shout out to Loïc Steiner for his amazing work in the lab (and his BBQ skills).

The initial idea for Bio LOGIC came from Ari Sarfatis.

The wiki was designed by Axel Uran. All team members contributed to the content of the wiki.

The establishment of the model of the project was done by Rocco Meli and Ari Sarfatis. The software implementation was done by Rocco Meli.

The Interlab study was conducted by Victor Steininger.

The Human Practices Article was written by Cyril Pulver. The interviews were done by Joseph Heng, Vincent Jacquot, Paola Malsot, Cyril Pulver, Loïc Steiner, Victor Steininger, Grégoire Thouvenin and Axel Uran.

The 'gymnase day' was mostly organized Vincent Jacquot and Victor Steininger, and all team members participated during the different activities.

The survey in Lausanne was done by Joseph Heng, Vincent Jacquot, Cyril Pulver, Victor Steininger, Grégoire Thouvenin. The survey in Basel with the team from Zurich Loïc Steiner and Victor Steininger.

Graphics were done by Emilie Cuillery.

Our advisors, Riccardo Dainese and Michael Frochaux, PhD students in Bart Deplancke's lab, and Ted Baldwin, Romane Breysse, Nikolaus Huwiler, Sakura Nussbaum and Cécile Piot, EPFL iGEM 2014 team members, helped us get started in the lab and were available to answer our questions and help us out during the project.

Our instructors, Bart Deplancke, Barbara Grisoni and Sebastian Maerkl, contributed support to help us solve some of the issues we encountered and go forward with our project, and showed us the path of scientific rigor.

The team members, from left to right: Paola Malsot, Grégoire Thouvenin, Vincent Jacquot, Loïc Steiner, Cyril Pulver, Joseph Heng, Emilie Cuillery, Victor Steininger, Rocco Meli, Ari Sarfatis, Nina van Tiel, Axel Uran
The (almost) full team with advisors and instructors, from left to right: Ted Baldwin, Barbara Grisoni, Bart Deplancke, Romane Breysse, Nikolaus Huwiler, Michael Frochaux, Vincent Jacquot, Cécile Piot, Sakura Nussbaum, Grégoire Thouvenin, Rocco Meli, Victor Steininger, Paola Malsot, Axel Uran, Emilie Cuillery, Loïc Steiner, Joseph Heng, Ari Sarfatis, Cyril Pulver, Nina van Tiel, Riccardo Dainese. Absent: Sebastian Maerkl.

Acknowledgements

We would like to acknowledge all the help and support we received throughout our iGEM adventure and would like to thank everyone who helped in any possible way and together made our project possible.

General support

A warm thanks to EPFL, the Swiss Federal Institute of Technology in Lausanne, whithout which our participation in iGEM and our project would not have been possible. We would like to thank André Pexieder for letting us use his lab and its equipment during the project, Marie-France Radigois who helped us with the administrative tasks related to the project and Stéphane Karlen, Safety coordinator at the School of Life Science, who gave us a course on laboratory safety before our project started and was available for any question during the project.

Lab and project support

Special thanks to David Bikard, from the Institut Pasteur, for sending us E. Coli strains JEN202, JEN202+pWJ89 and DH5a+pWJ66, a central part for the wet lab in E. Coli.
We would like to thank Andreas Mayer's lab from the University of Lausanne (UNIL) for giving us the strain of S. cerevisiae W303 for our experiments.
We would like to thank Miguel Garcia who taught us the basics of flow cytometry and the EPFL Flow Cytometry Core Facility for letting us use their flow cytometer. Thanks to Andre Mozes and Loïc Tauzin for assisting us during our measurements.
We would also like to thank Marc Chambon and Henrike Niederholtmeyer who taught us how to use the plate reader and Sebastian Maerkl for letting us use the plate reader in his lab.
And thanks to Julie Russeil who helped us through our first successful yeast integration and gave us useful tips for working with yeast.
Special thanks to Paolo De Los Rios, head of the Laboratory of Statistical Biophysics, for a very useful discussion on our model, his advice and constuctive inputs.
Thanks to Sahar Hosseinian Ehrensberger for helping us with statistics concerning the Interlab study.

Human Practices Article

We would like to thank the experts that took the time to meet us and share their opinion and experience in their respective domains. Below is a list of these experts in alphabetical order:

  • Gaia Barazzetti - Ethicist, researcher at University of Lausanne, specialist in bioethics
  • Lazare Benaroyo - Ethicist, researcher in ethics linked with genomics at University of Lausanne
  • Aurélie Coulon - Journalist at Le Temps, section Science
  • Denis Duboule - Scientist, researcher at EPFL, specialist in embryology
  • Delphine Ducoulombier - Scientific mediator at l'Eprouvette, the public laboratory of University of Lausanne
  • Xavier Gravend - Theologist, catholic chaplain at EPFL
  • François Lefort - Scientist, researcher in agronomical microbiology at hepia HES-SO, member of the Parliament of the State of Geneva
  • Jean-Christophe Méroz - Legislation division at Swissmedic, Federal agency for therapeutic products
  • Oliver Peter - Scientist, head of the high-throughput screening group at Actelion Pharmaceuticals
  • Didier Trono - Scientist, researcher at EPFL, specialist in virology
  • Christian Vez - Pastor, protestant chaplain at EPFL

Thanks to Jamani Caillet for letting us use his photography as an illustration in the article.

Sponsors

We would like to thank all our generous sponsors.

EPFL

EPFL is one of the two Swiss Federal Institutes of Technology and is located in Lausanne, Switzerland. This school was founded with the stated mission to educate engineers and scientists, to be a national center of excellence in science and technology and to provide a hub for interaction between the scientific community and industry. It is our main sponsor.

F. Hoffmann-La Roche is a Swiss global health-care compagny hearquartered in Basel, Switzerland, that operates worldwide under two divisions : Pharmaceuticals and Diagnostics.

Nikon

Nikon is a Japanese multinational corporation headquartered in Tokyo, Japan, specializing in optics and imaging products, including cameras, camera lenses, binoculars, microscopes, ophtalmic lenses, measurement instruments, etc.

SnapGene is a molecular biology software that offers a fast and easy way to plan, visualize and document molecular biology procedures.

SnapGene
Microsynth

Microsynth is a European leader in offering services related to synthesis of DNA/RNA oligonucleotides, various kinds of DNA/RNA analyses and molecular biology project outsourcing.

Novartis is a Swiss multinational pharmaceutical compagny based in Basel, Switzerland, ranking number one in sales amog the world-wide industry in 2013.

Novartis
New England Biolabs

New England Biolabs (NEB) produces and supplies recombinant and native enzyme reagents for the life science research and also provides free access to research tools such as REBASE, InBASE, and Polbase.

Integrated DNA Technologies (IDT) is a leader in development and manufacturing of products for research and diagnostic life science markets. It is the world’s largest supplier of custom nucleic acids and serves academic research, biotechnology, and pharmaceutical development communities.

Integrated DNA Technologies
EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits