Team:Groningen/Notebook/sinR Transformation JH642 si1
Blue Bio Energy
Transformation of SinR in JH642
overexpression of SinR
Overexpression of SinR in Bacillus subtilis strains. This was done to overexpress matrix genes in the strains, which should result in an increased robustness of their biofilm.
failed
Transformation
15:48, 24 August 2015 - 24:00, 24 August 2015
MC was placed in the 37°C incubator. The OD was measured at 20:30 but not grown far enough.1 µL of SinR DNA was added to 200 µL of MC with JH642. The culture was plated at 24:00.
18:00, 25 August 2015 - 24:00, 25 August 2015
No colonies were seen so the transformation failed