Team:Marburg/Protocols/Protocol24
Zymo DNA Clean & Concentrator™-5
- In a 1.5 ml microcentrifuge tube, add 2-7 volumes of DNA Binding Buffer to each volume of DNA sample (see table below). Mix briefly by vortexing.
- Transfer mixture to a provided Zymo-Spin™ Column in a Collection Tube.
- Centrifuge for 30 seconds. Discard the flow-through.
- Add 200 µl DNA Wash Buffer to the column. Centrifuge for 30 seconds. Repeat the wash step.
- Add ≥ 6 µl DNA Elution Buffer or water directly to the column matrix and incubate at room temperature for one minute. Transfer the column to a 1.5 ml microcentrifuge tube and centrifuge for 30 seconds to elute the DNA.
| Application | DNA Binding Buffer : Sample |
|---|---|
| Plasmid, genomic DNA (>2 kb) | 2:1 |
| PCR product, DNA fragment | 5:1 |
| ssDNA | 7:1 |
