Zymo DNA Clean & Concentrator™-5

  • In a 1.5 ml microcentrifuge tube, add 2-7 volumes of DNA Binding Buffer to each volume of DNA sample (see table below). Mix briefly by vortexing.

  • Application DNA Binding Buffer : Sample
    Plasmid, genomic DNA (>2 kb) 2:1
    PCR product, DNA fragment 5:1
    ssDNA 7:1

  • Transfer mixture to a provided Zymo-Spin™ Column in a Collection Tube.
  • Centrifuge for 30 seconds. Discard the flow-through.
  • Add 200 µl DNA Wash Buffer to the column. Centrifuge for 30 seconds. Repeat the wash step.
  • Add ≥ 6 µl DNA Elution Buffer or water directly to the column matrix and incubate at room temperature for one minute. Transfer the column to a 1.5 ml microcentrifuge tube and centrifuge for 30 seconds to elute the DNA.
iGEM Marburg - ZSM Karl-von-Frisch-Straße 16, D - 35043 Marburg