Team:Marburg/Protocols/Protocol5
CPEC Standard
Mix:- 10 µL DNA
- 10 µL 5x GXL Buffer
- 4 µL dNTP Mix
- 24 µL ddH2O
- 2 µL GXL Polymerase
Program:
- 98.0 °C for 30 s
- Cycle (25x):
- 98.0 °C for 15 s
- 55.0 °C (0.1 °C/s) for 30 s
- 72.0 °C for 3 min
- 72.0 °C for 7 min
- Store @ 8.0 °C
