Team:UCLA/Notebook/Protein Cages/31 July 2015
Nithin's Notes
I ran a touchdown pcr today. There is a strong band where we want it and a weak nonspecific band. Will upload pic tmro.
Prefix 1 and Suffix 1 with PCquad.
Conditions:
- 1-cycle-
- 98 degrees- 30s
- 10-cycles
- - 98 degrees- 10s
- - 76-66 degrees (decrease by 1 degree each cycle)-30 seconds
- - 72 degrees- 15s
- 15-cycles
- - 98 degrees- 10s
- - 66 degrees- 30s
- - 72 degrees- 15s
- 1-cycle
- - 72 degrees- 5 minute
- - 4 degree hold.