Team:UCLA/Notebook/Spider Silk Genetics/15 April 2015

iGEM UCLA

4/15/2015

Set up two 50 uL PCR reactions each for 2BC and 2CA using the same guidelines on 4/13/2015 with the following modifications:

∙ Annealing Temp: 64 C

∙ 25 cycles instead of 20

∙ Visualized with 1.5% agarose gel. Similar result to yesterday’s gel.

∙ Used gel purification kit (zymo) to extract DNA from 2AB, 2BC, and 2CA.