Template:Team:Groningen/CONTENT/LOGBOOK/Transform with GFP py14
Transformation of Bacillus subtilis PY79 with GFP
Investigates whether Bacillus subtilis PY79 can be transformed with GFP containing plasmid.
Investigating Bacillus subtilis PY79 submerged biofilm growth.
No green fluorescent colonies were found. Therefore, Bacillus subtilis PY79 is not able to be transformed with this GFP plasmid.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Transformation">Transformation</a>
11:00, 22 Augustus 2015 - 00:00, 22 Augustus 2015
Standard competence medium was prepared for Bacillus subtilis PY79.
Ingredient
Protocol
Actual
H20
1.8 mL
1.71 mL
MC 10x
200 µL
200 µL
MgSO4 (1M)
6.7 µL
6.7 µL
Trp 1%
10 µL
97.9 µL of 5mM
Ingredients for standard competence medium
Bacillus subtilis PY79 was added to standard competence medium.
Measured optical density of Bacillus subtilis PY79 culture 10 times diluted in demiwater: 0.111.
5 µL of GFP spectinomycin DNA was added to diluted Bacillus subtilis PY79 culture
Plated Bacillus subtilis PY79 on spectinomycin plates.
09:00, 23 Augustus 2015 - 00:00, 23 Augustus 2015
Clear colonies were visible on the plate. Unfortunately, the colonies were not very green.