Difference between revisions of "Team:Nagahama/Medal Parts"
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The decane overlay in the two-phase culture did not affect growth, and FOH could be solubilized in the decane phase with negligible volatile loss. | The decane overlay in the two-phase culture did not affect growth, and FOH could be solubilized in the decane phase with negligible volatile loss. | ||
| − | We | + | We adopted 1 mL of decane overlaid to 5 mL of culture broth. |
Revision as of 09:55, 9 September 2015
Contents
Medal parts
銅
銀
λPL+r.b.s.+ispA＋MEP+×× (r.b.s.+dxs+r.b.s.+m-idi+r.b.s.+ispDF)
FOH is probably generated through FPP hydrolysis by endogenous phosphatases, which are induced by an increased intracellular FPP level (Asadollahi et al., 2008; Muramatsu et al., 2008a).
Analogously, we hypothesized that E. coli could produce FOH under cellular conditions of an increased intracellular FPP level through metabolic engineering.
A MEP pathway has been shown to synthesize IPP and DMAPP efficiently in E. coli (Martin et al., 2003).
Because of its high hydrophobicity and low volatility, decane was chosen to extract and solubilize FOH from culture broth.
The decane overlay in the two-phase culture did not affect growth, and FOH could be solubilized in the decane phase with negligible volatile loss.
We adopted 1 mL of decane overlaid to 5 mL of culture broth.
Gas Chromatography/Mass(GC/MS)
菌
Note
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Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.