Difference between revisions of "Team:Czech Republic/Notebook"
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Verification of the pRSII416_ste2 construct ([[Team:Czech_Republic/Protocols#Miniprep|Miniprep]], [[Team:Czech_Republic/Protocols#Restriction_digest|Restriction digest]], [[Team:Czech_Republic/Protocols#Gel_electrophoresis|Gel electrophoresis]]). | Verification of the pRSII416_ste2 construct ([[Team:Czech_Republic/Protocols#Miniprep|Miniprep]], [[Team:Czech_Republic/Protocols#Restriction_digest|Restriction digest]], [[Team:Czech_Republic/Protocols#Gel_electrophoresis|Gel electrophoresis]]). | ||
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+ | [[File:Czech_Republic_M2_Ste2verif.png|250px]] | ||
== Week 2 (11-17) == | == Week 2 (11-17) == |
Revision as of 08:11, 10 September 2015
Notebook
Contents
May
Week 1 (4-10)
Module 2
PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain (Genome extraction, PCR, Gel electrophoresis).
Cloning of the amplified ste2 into pRSII416 vector. (Restriction digest, Ligation, Transformation).
Verification of the pRSII416_ste2 construct (Miniprep, Restriction digest, Gel electrophoresis).
Week 2 (11-17)
Week 3 (18-24)
Week 4 (25-31)
June
Week 5 (1-07)
Week 6 (08-14)
Week 7 (15-21)
Week 8 (22-28)
Week 9 (29-05)
July
Week 10 (06-12)
Week 11 (13-19)
Week 12 (20-26)
Week 13 (27-02)
August
Week 14 (03-09)
Week 15 (10-16)
Week 16 (17-23)
Week 17 (24-30)
Week 18 (31-06)
September
Week 19 (07-13)
Week 20 (14-20)