Difference between revisions of "Team:Czech Republic/Notebook"

(Module 2)
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Verification of the pRSII416_ste2 construct ([[Team:Czech_Republic/Protocols#Miniprep|Miniprep]], [[Team:Czech_Republic/Protocols#Restriction_digest|Restriction digest]], [[Team:Czech_Republic/Protocols#Gel_electrophoresis|Gel electrophoresis]]).
 
Verification of the pRSII416_ste2 construct ([[Team:Czech_Republic/Protocols#Miniprep|Miniprep]], [[Team:Czech_Republic/Protocols#Restriction_digest|Restriction digest]], [[Team:Czech_Republic/Protocols#Gel_electrophoresis|Gel electrophoresis]]).
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[[File:Czech_Republic_M2_Ste2verif.png|250px]]
  
 
== Week 2 (11-17) ==
 
== Week 2 (11-17) ==

Revision as of 08:11, 10 September 2015

Notebook

May

Week 1 (4-10)

Module 2

PCR of the ste2 gene from the genome of Saccharomyces cerevisiae 7284 strain (Genome extraction, PCR, Gel electrophoresis).

Czech Republic M2 gel-05-04.png

Cloning of the amplified ste2 into pRSII416 vector. (Restriction digest, Ligation, Transformation).

Verification of the pRSII416_ste2 construct (Miniprep, Restriction digest, Gel electrophoresis).

Czech Republic M2 Ste2verif.png

Week 2 (11-17)

Week 3 (18-24)

Week 4 (25-31)

June

Week 5 (1-07)

Week 6 (08-14)

Week 7 (15-21)

Week 8 (22-28)

Week 9 (29-05)

July

Week 10 (06-12)

Week 11 (13-19)

Week 12 (20-26)

Week 13 (27-02)

August

Week 14 (03-09)

Week 15 (10-16)

Week 16 (17-23)

Week 17 (24-30)

Week 18 (31-06)

September

Week 19 (07-13)

Week 20 (14-20)