Difference between revisions of "Team:Technion HS Israel/Notebook"

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<link href="//2015.igem.org/Template:Technion_HS_Israel4/Technion_HS_Israel_menu_style?action=raw&ctype=text/css" rel="stylesheet">
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<h1>Notebook</h1>
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<p> Here is a summery of our notebook. For the full notebook please look at the following scans</p> 
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<table class="NewTable"    border="1">
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<thead>
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<tr>
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  <th>Week</th>
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  <th>Design</th>
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  <th>Cloning</th>
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  <th>Experiment</th>
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  <th>Finish new BioBrick</th>
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</tr>
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</thead>
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<tr>
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  <td>Jul 5th to Jul 9th (2015)</td>
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  <td>Primers : OGEM 1 and 2 to open j09855 plasmid . <br></br>Gblock :RBS- Aiia with a Gibson overlabs.</td>
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  <td>we got j09855 part from the distribution kit.</td>
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  <td>-</td>
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  <td>-</td>
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</tr>
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<tr>
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  <td>June 12th to July 16th (2015)</td>
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  <td>Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids.  </td>
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  <td>PCR to open the j09855 plasmid.Gibson to add Aiia .</td>
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  <td>-</td>
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  <td>BBa_K1767001</td>
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</tr>
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<tr>
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  <td>Aug. 2nd to Aug. 6th (2015)</td>
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  <td>-</td>
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  <td>We got p0440 part from the distribution kit.<br></br>We built BBa_K1767002 and BBa_K1767004  Constructs by restriction enzymes. <br></br>PCR to get the k1767001 plasmid without LuxR .</td>
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  <td>-</td>
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  <td>BBa_K1767002 <br></br>BBa_K1767004</td>
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</tr>
  
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<h2>Notebook</h2>
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<tr>
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  <td>Aug. 9th to Aug. 13th (2015)</td>
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  <td>Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR)</td>
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  <td>We got I13521 part from the distribution kit.</br> We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes. </br>Colony PCR to check if we have LuxR in the plasmid.</td>
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  <td>-</td>
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  <td>BBa_K1767003 </br>BBa_K1767005</td>
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</tr>
  
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<p> Document the dates you worked on your project.</p>
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<tr>
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  <td>Aug. 16th to Aug. 20th (2015)</td>
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<h5>What should this page have?</h5>
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  <td>-</td>
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<ul>
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  <td>-</td>
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<li>Chronological notes of what your team is doing.</li>
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  <td>AHL experiment #1</td>
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<li> Brief descriptions of daily important events.</li>
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  <td>-</td>
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<li>Pictures of your progress. </li>
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</tr>
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<li>Mention who participated in what task.</li>
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<tr>
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</ul>
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  <td>Aug. 23rd to Aug. 27th (2015)</td>
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  <td>Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ).</td>
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  <td>We got I13003 part from the distribution kit.</td>
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<h4>Inspiration</h4>
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  <td>AHL experiment #2</td>
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<p>You can see what others teams have done to organize their notes:</p>
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  <td>-</td>
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</tr>
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<ul>  
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<tr>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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  <td>Aug. 30th to Sept. 9th (2015)</td>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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  <td>Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA )</td>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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  <td>We built BBa_K1767009 and BBa_K1767010  Constructs by restriction enzymes.</br>Colony PCR to check if we have YFP in the plasmid.</td>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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  <td>-</td>
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</ul>
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  <td>BBa_K1767009 </br>BBa_K1767010
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</td>
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</tr>
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<tr>
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  <td>Sept. 6th to Sept. 11th (2015)</td>
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  <td>-</td>
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  <td>-</td>
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  <td>AHL experiment # 3</td>
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  <td>-</td>
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</tr>
  
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</table>
 
</div>
 
</div>
 
</html>
 
</html>

Revision as of 09:22, 12 September 2015

Technion 2015 HS Team's Wiki

Notebook

Here is a summery of our notebook. For the full notebook please look at the following scans

Week Design Cloning Experiment Finish new BioBrick
Jul 5th to Jul 9th (2015) Primers : OGEM 1 and 2 to open j09855 plasmid .

Gblock :RBS- Aiia with a Gibson overlabs.		 we got j09855 part from the distribution kit. - -
June 12th to July 16th (2015) Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. PCR to open the j09855 plasmid.Gibson to add Aiia . - BBa_K1767001
Aug. 2nd to Aug. 6th (2015) - We got p0440 part from the distribution kit.

We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes.

PCR to get the k1767001 plasmid without LuxR .		 - BBa_K1767002

BBa_K1767004
Aug. 9th to Aug. 13th (2015) Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) We got I13521 part from the distribution kit.
We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes.
Colony PCR to check if we have LuxR in the plasmid.		 - BBa_K1767003
BBa_K1767005
Aug. 16th to Aug. 20th (2015) - - AHL experiment #1 -
Aug. 23rd to Aug. 27th (2015) Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). We got I13003 part from the distribution kit. AHL experiment #2 -
Aug. 30th to Sept. 9th (2015) Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.
Colony PCR to check if we have YFP in the plasmid.		 - BBa_K1767009
BBa_K1767010
Sept. 6th to Sept. 11th (2015) - - AHL experiment # 3 -