Difference between revisions of "Team:Bielefeld-CeBiTec/Results/HeavyMetals"

Line 131: Line 131:
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://2015.igem.org/File:Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://2015.igem.org/File:Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
Line 137: Line 137:
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://2015.igem.org/File:Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://2015.igem.org/File:Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/c/cf/Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/cf/Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
Line 143: Line 143:
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://2015.igem.org/File:Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://2015.igem.org/File:Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://2015.igem.org/File:Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://2015.igem.org/File:Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
Line 160: Line 160:
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
Line 166: Line 166:
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/4/41/Bielefeld-CeBiTec_Biolector_nickel_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/41/Bielefeld-CeBiTec_Biolector_nickel_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>

Revision as of 02:13, 14 September 2015

iGEM Bielefeld 2015


Heavy Metals

Zusammenfassung in ganz wenigen Worten.


The different sensors we worked with were characterized in vivo as well as in vitro.

We tested the influence of each heavy metal on our sensors in vivo Therefore we used heavy metal concentrations based on heavy metal occurrences measured all over the world.
Adjusting the detection limit
Influence of heavy metals on the growth of E.coli KRX shown is the standard deviation of three biological replicates. For induction concentrations of 20 µg/L lead, 60 µg/L mercury, 60 µg/L chromium, 80 µg/L nickel, 40 mg/L copper which represent ten times of the WHO guideline were used.

After finding out that the tested concentrations had no negative effect on E. colis growth we started in vivo characterization

in vivo

Arsenic

Adjusting the detection limit
Time course of the induction of an arsenic biosensor with RFP for different arsenic concentrations in vivo. Error bars represent the standard deviation of three biological replicates.

Chromium

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Copper

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Lead

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Mercury

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Nickel

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

in vitro

Arsenic

Chromium

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Copper

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Mercury

And the date rape drugs?