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Revision as of 09:13, 14 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Practices

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Highschool students' visit

Start of the day

Since iGEM is encouraging young students to get into the lab and engage in innovative ideas using Synthetic Biology, our aim for this day was to get this mindset across to high school students. In order to do so, we contacted three different high schools in our region. [insert name of highschool], [insert name of highschool] and [insert name of highschool] keenly sent us students interested in biology and eager to find out about synthetic biology.

Indeed, we discovered that, even if the level of biology is fairly high in Switzerland, the material taught does not include Synthetic Biology. At the end of the day, we strove so that these students would have a new view of the field of biology and that even engineers should be interested in this field, that we can hack life!

Joseph_Cyril_Nina depart
Cyril Presentation

Presentation

We welcomed the students with a presentation which consisted of three parts. The first one was about iGEM, the modalities and principles of the competition, the number of participating teams, the contribution of each team, etc. The second part was about Synthetic Biology and, after introducing them about common lab techniques and procedures, we talked about the third part, our « BioLOGIC » project.

After the presentation we dispatched them into three groups for three different activities


mini_iGEM
mini_iGEM
mini_iGEM

Mini iGEM

As we wanted them to truly understand the iGEM competition we held a mini iGEM competition. In this activity, small groups of students worked together to create iGEM projects they would present to other teams. They were asked to think about feasibility, utility, safety and ethics. During the brainstorming, each team was coached by one of our team. We introduced the concepts of kill switch, biobricks, motility, transcription pathways, transporters and bio-informatics to them. The ideas that they shared with us were surprisingly smart and imaginative for students who had received little technical background information on the matter. Some teams even brought up and discussed projects that had already been realised in previous iGEM competitions.

Lab immersion

Even the most renowned scientist started by pipetting water, this is why we prepared a few activities in the lab to let the students see how it is to work in the world of synthetic biology.[insert activities]

Ethics debate

Lastly, ethics is really important in the field of Sciences. The misuse of novel technologies is a matter that every scientist with integrity has to think about. To make the students reflect about actions and consequences we prepared an ethics debate during which we gave the students a role to defend. We gave them these roles because we wanted them to be able to think about another opinion and it was great to see them get into their role and having fun doing so. At the end of the debate we exited the roles and were pleased to see that what we had opened their eyes on different aspects concerning the responsibility that we have concerning the research. [maybe talk about swiss votation system?]

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Mini iGEM projects

Name Description
Famousse Famousse ("Mousse" means foam in french) is a gun that sprays an isolating material to fill walls. The fluid consists of bacterias that produce foam once in contact with oxygen and of yeasts that make CO2 holes in the foam. After a while, the foam solidifies and bacterias and yeasts die by apoptosis: the isolating material is finished.
RIDG pill The RIDG pill (Régulateur intelligent Des Graisses or Intelligent Fat Regulator, IFR pill) is made out of bacteria that detect, once in the intestine, if the person suffers of obesity or anorexia thanks to hormone receptors. In case of obesity, the bacteria would respond to that input by secreting proteins that block lipid transporters in epithelial cells. In case of anorexia, the bacteria would secrete hormones that would make epithelial cells create more lipid transporters.
PestiBac Bacteria substituting pesticides. These bacteria live on the leaves of the plants and secrete repelling substances. These bacteria have sensor in order to die if not in contact with the target plant or if they came in contact with animal's saliva. In addition they are modified to have a shorter lifetime, in order to limit population growth.
Luciolight Luciolight is a Street Lamp made out of bacteria that copy biological circuit of fireflies.The bacteria would produce the light-emitting luciferin, thanks to the two proteins luciferase and phosphoprotein. The team thought of reproducing also the system of light-emitting fishes, deep in th sea. This would produce light without electricity.
Bacteriolavage Bacteriolavage is a bacterial washing system to clean clothes with less water waste. Bacteria synthesize enzymes that degrade lipids and sugars in smaller particles that are more water-soluble, so that less water is consumed. Students used the concept of magnetic bacteria developed by the 2014 iGEM Berlin team to efficiently remove bacterias from clothes once the wash was finished.
Killpilus Killpilus is a permanent hair removal cream. Bacteria contained in the preparation kill hair follicle stem cells. The team searched the internet for receptors specifically expressed in those stem cells to avoid off targets. For safety reasons, bacteria would commit suicide by apoptosis after one hour.
ConchitaColi ConchitaColi cells digest dust and produce scented molecules. After the washing is finished, bacterias move towards a luminous bin where they die by apoptosis.
Healing+ "Healing+" is a system to repair wounds. Mammalian cells circulating in the blood detect intrusion of pathogens and respond by mobilisation of collagen and platelets in wounds.
easyFer "easyFer" ("easyIron") is a system that uses an iron detecting biosensor to simplify the iron detection in the blood.
EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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