Difference between revisions of "Team:Bielefeld-CeBiTec/Results/HeavyMetals"
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</br></br> | </br></br> | ||
+ | <!-- Einfluss von Kupfer auf den Zellextrakt --> | ||
<figure style="width: 600px"> | <figure style="width: 600px"> | ||
<a href="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="Influence of different copper concentrations on our crude cell extract. Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a> | <a href="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="Influence of different copper concentrations on our crude cell extract. Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a> | ||
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</figure> | </figure> | ||
− | + | <!-- Induktion mit Kupfer im Kupfer spezifischen Extrakt --> | |
<figure style="width: 600px"> | <figure style="width: 600px"> | ||
<a href="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="Copper specific cell extract made from <i>E. coli</i> cells which have already expressed the activator before cell extract production. Induction of copper inducible promoter without T7 in front of the operator site with different copper concentrations. Error bars represent the standard deviation of three biological replicates. "><img src="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> | <a href="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="Copper specific cell extract made from <i>E. coli</i> cells which have already expressed the activator before cell extract production. Induction of copper inducible promoter without T7 in front of the operator site with different copper concentrations. Error bars represent the standard deviation of three biological replicates. "><img src="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> | ||
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</figure> | </figure> | ||
− | + | <!--obrige Abbildung durch den errechneten Korrekturfaktor angepasst, da verschiedene Faktoren auf Zellextrakt wirken und so diesen beeinflussen.--> | |
<figure style="width: 600px"> | <figure style="width: 600px"> | ||
<a href="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="Copper specific cell extract made from <i>E. coli</i> cells which have already expressed the activator before cell extract production. Induction of copper inducible promoter without T7 in front of the operator site with different copper concentrations. Error bars represent the standard deviation of three biological replicates. Data are normalized on coppers influence to the cell extract."><img src="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> | <a href="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="Copper specific cell extract made from <i>E. coli</i> cells which have already expressed the activator before cell extract production. Induction of copper inducible promoter without T7 in front of the operator site with different copper concentrations. Error bars represent the standard deviation of three biological replicates. Data are normalized on coppers influence to the cell extract."><img src="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> | ||
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</figure> | </figure> | ||
+ | <!-- Es wurde auch das Konstrukt mit einen T7 davor eingesetzt, es zeichen sich unterschhiede inder Flurescens ausbeute, so mit ist für das CFPS system ein vorgeschalteter T7 sinnvoll zur besseren sensitivität des Systems. --> | ||
<figure style="width: 600px"> | <figure style="width: 600px"> | ||
<a href="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="Copper specific cell extract made from <i>E. coli</i> cells which have already expressed the activator before cell extract production. Induction with different copper concentrations. Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> | <a href="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="Copper specific cell extract made from <i>E. coli</i> cells which have already expressed the activator before cell extract production. Induction with different copper concentrations. Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> | ||
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</figure> | </figure> | ||
+ | <!-- auch dieses Abbildung wurde mit dem Korrekturfaktor korrigiert--> | ||
<figure style="width: 600px"> | <figure style="width: 600px"> | ||
<a href="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> | <a href="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a> |
Revision as of 12:40, 14 September 2015
Heavy Metals
Zusammenfassung in ganz wenigen Worten.
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/7/7d/Bielefeld-CeBiTec_heavy_metal_influence_growth.jpeg)
Arsenic
in vivo
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/2/2e/Bielefeld-CeBiTec_Arsenic_invivo_small.png)
in vitro
Chromium
in vivo
Our sensor for chromium detection consists of ChrB the repressor and the chromate specific promoter ChrP. The promoter is regulated by the ChrB, which binds Cr-ions. Behind the promoter is a sfGFP for detection of a fluorescence signal. In vivo we could show that the addition of different concentrations of chromium have different effects to transcription of sfGFP.![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/8/82/Bielefeld-CeBiTec_Biolector_chromium.jpg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/7/73/Bielefeld-CeBiTec_Biolector_chromium_Balkendiagramm.jpg)
in vitro
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/9/99/Bielefeld-CeBiTec_Influence_of_chromium_on_the_cell_extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/d/d0/Bielefeld-CeBiTec_induction_chromium_in_chrB-cell-extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/1/1e/Bielefeld-CeBiTec_correction_induction_chromium_in_chrB-cell-extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/1/1d/Bielefeld-CeBiTec_induction_chromium_in_chrB_optimized_cell-extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/f/fe/Bielefeld-CeBiTec_Corr-induction-Cr-in-ChrBopt-CE.jpeg)
Copper
in vivo
Our sensor for copper detection consists of CueR a MerR like activator and the copper specific promoter CopAP. The promoter is regulated by CueR, which binds Cu2+-ions. We also used a sfGFP behind the promoter for detection trough a fluorescence signal.![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg)
in vitro
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg)
Lead
in vivo
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/d/d5/Bielefeld-CeBiTec_Biolector_lead.jpg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/a/aa/Bielefeld-CeBiTec_Biolector_lead_Balkendiagramm.jpeg)
Mercury
in vivo
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/5/5b/Bielefeld-CeBiTec_mercury_flourescence-in_vivio.png)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/5/52/Bielefeld-CeBiTec_mercury_fluorescence_in_vivo_Balkendiagramm.jpeg)
in vitro
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/b/b9/Bielefeld-CeBiTec_Influence_of_mercury_on_the_cell_extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/1/1e/Bielefeld-CeBiTec_Abnahme_unter_Hg.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/7/7f/Bielefeld-CeBiTec_induction_mercury_in_merR_cell-extract.jpeg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/f/f9/Bielefeld-CeBiTec_correction_induction_mercury_in_merR_cell-extract.jpeg)
Nickel
in vivo
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/c/c9/Bielefeld-CeBiTec_Biolector_nickel.jpg)
![Adjusting the detection limit](https://static.igem.org/mediawiki/2015/4/41/Bielefeld-CeBiTec_Biolector_nickel_Balkendiagramm.jpeg)