Difference between revisions of "Team:Mingdao/Design"

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{{Mingdao}}
 
 
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<h2>Design</h2>
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<script type="text/javascript">
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  <meta name="generator" content="2014.0.0.264"/>
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  <title>Design</title>
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  <!-- CSS -->
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  <link rel="stylesheet" type="text/css" href="css/site_global.css?84057463"/>
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  <link rel="stylesheet" type="text/css" href="css/master_a-master.css?181564128"/>
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  <link rel="stylesheet" type="text/css" href="css/design.css?4211058826" id="pagesheet"/>
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  document.documentElement.className += ' js';
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  <script src="scripts/html5shiv.js?4241844378" type="text/javascript"></script>
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    <!--custom head HTML-->
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  #menuu6995{
  
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By talking about your design work on this page, there is one medal criterion that you can attempt to meet, and one award that you can apply for. If your team is going for a gold medal by building a functional prototype, you should tell us what you did on this page. If you are going for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Applied Design award</a>, you should also complete this page and tell us what you did.  
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Applied Design award</a> and/or the <a href="https://2015.igem.org/Judging/Awards#Medals">functional prototype gold medal criterion</a>, you must fill out this page.</p>
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<p>This is a prize for the team that has developed a synthetic biology product to solve a real world problem in the most elegant way. The students will have considered how well the product addresses the problem versus other potential solutions, how the product integrates or disrupts other products and processes, and how its lifecycle can more broadly impact our lives and environments in positive and negative ways.</p>
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        <img class="colelem" id="u3831-4" alt="Prototype" width="815" height="102" src="images/u3831-4.png"/><!-- rasterized frame -->
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        <div class="clearfix colelem" id="u3833-23"><!-- content -->
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          <p>After finding, analyzing and testing with our novel fire retardant bio&#45;material of phosphorylated SR protein, we would like to challenge the effectiveness of our fire retardant bio&#45;coatings on real products. Fire retardant clothes and fire retardant paint attract increasingly interest in the market and industry. We applied our prototypes of fire retardant bacteria which produce phosphorylated SR proteins to mixing cotton and painting on a model house.</p>
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          <p>&nbsp;</p>
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          <p>Fire retardant bacteria were made as described in the procedure of protein inducation and analysis as well as flame test.</p>
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          <p>↓ Recover bacteria carrying genes of SR and SRPK1 proteins from &#45;80°C stock</p>
 +
          <p>↓ Culture at 37°C O/N</p>
 +
          <p>↓ Dilute 100X with fresh media and incubate for 2 hr till OD between 0.6~1.0</p>
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          <p>↓ Add 1mM IPTG to induce protein expression for 4 hr</p>
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          <p>↓ Soak cotton in the culture media containing bacteria</p>
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          <p>Or ↓ Centrifuge the bacteria and paint on the house</p>
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          <p>↓ Dry the bacteria completely in the oven at 100°C over 30 min</p>
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          <p>↓ Subjected to flame test with gas fire gun as indicated condition</p>
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    <nav class="MenuBar clearfix pinned-colelem" id="menuu6995"><!-- vertical box -->
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    <div class="MenuItemContainer clearfix colelem" id="u7031"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7032" href="project.html#motivation"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7034-4"><!-- content --><p id="u7034-2">Motivation&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2</p></div></a>
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    <div class="MenuItemContainer clearfix colelem" id="u7010"><!-- horizontal box -->
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    <div class="MenuItemContainer clearfix colelem" id="u7017"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7018" href="project.html#result"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7019-4"><!-- content --><p id="u7019-2">Results&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 5</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7024"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7027" href="project.html#protein-database-mining"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7029-4"><!-- content --><p id="u7029-2">&nbsp; Protein Database Mining&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7059"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7060" href="project.html#fire-retardant-candidate"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7061-4"><!-- content --><p id="u7061-2">&nbsp; Fire Retardant Candidate&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7115"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7118" href="project.html#gene-cloning"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7121-4"><!-- content --><p id="u7121-2">&nbsp; Gene Cloning&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    <div class="MenuItemContainer clearfix colelem" id="u7052"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7053" href="project.html#protein-expression"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7054-4"><!-- content --><p id="u7054-2">Protein Expression&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7038"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7039" href="project.html#flame-test"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7041-4"><!-- content --><p id="u7041-2">Flame Test&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7066"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7067" href="project.html#summary-discussion"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7070-4"><!-- content --><p id="u7070-2">Summary &amp; Discussion&nbsp;&nbsp;&nbsp; 6</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7087"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7088" href="design.html#prototype"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7091-4"><!-- content --><p id="u7091-2">Prototype&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 7</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7003"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7006" href="design.html#cotton"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7009-4"><!-- content --><p id="u7009-2">&nbsp;Cotton with Bio&#45;Coating&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u6996"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u6997" href="design.html#house"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u6999-4"><!-- content --><p id="u6999-2">&nbsp;House with Bio&#45;Coating&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7080"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7083" href="application.html#application"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7086-4"><!-- content --><p id="u7086-2">Application&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 8</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7129"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7130" href="application.html#fireretardantbio-paint"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7133-4"><!-- content --><p id="u7133-2">Fire Retardant Bio&#45;Paint&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7094"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7095" href="application.html#fireretardant-silk"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7096-4"><!-- content --><p id="u7096-2">Fire Retardant Silk&nbsp;&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </div>
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    <div class="MenuItemContainer clearfix colelem" id="u7108"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7109" href="collaboration.html"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7111-4"><!-- content --><p id="u7111-2">Collaboration&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 9</p></div></a>
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    <div class="MenuItemContainer clearfix colelem" id="u7045"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7048" href="collaboration.html#data-mining"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7049-4"><!-- content --><p id="u7049-2">Data mining&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    <div class="MenuItemContainer clearfix colelem" id="u7122"><!-- horizontal box -->
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      <a class="nonblock nontext MenuItem MenuItemWithSubMenu rgba-background clearfix grpelem" id="u7123" href="collaboration.html#biobrick-parts-validation"><!-- horizontal box --><div class="MenuItemLabel clearfix grpelem" id="u7126-4"><!-- content --><p id="u7126-2">BioBrick parts validation&nbsp;&nbsp;&nbsp; ●</p></div></a>
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    </nav>
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    <a class="anchor_item colelem" id="cotton"></a>
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    <div class="clearfix colelem" id="pu3834"><!-- group -->
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    <div class="clearfix grpelem" id="u3834"><!-- group -->
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      <div class="clearfix grpelem" id="u3835"><!-- group -->
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      <img class="grpelem" id="u3837-4" alt="Cotton" width="148" height="48" src="images/u3837-4.png"/><!-- rasterized frame -->
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      <div class="clearfix grpelem" id="u3836-4"><!-- content -->
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        <p>*</p>
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      </div>
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      </div>
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      <div class="clearfix grpelem" id="u3838-4"><!-- content -->
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      <p>*</p>
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      </div>
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    </div>
 +
    <div class="clearfix grpelem" id="u3839-6"><!-- content -->
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      <p>Samples: cotton (5X5 cm2)</p>
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      <p>Condition: Ignition till burning up (cotton control) or continues to the end (bio&#45;coating).</p>
 +
    </div>
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    </div>
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    <div class="clearfix colelem" id="pu4181-4"><!-- group -->
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    <div class="clearfix grpelem" id="u4181-4"><!-- content -->
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      <p>Cotton uncoated as control</p>
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    </div>
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    <div class="clearfix grpelem" id="u4183-4"><!-- content -->
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      <p>Cotton bio&#45;coated with fire retardant bacteria</p>
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    </div>
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    <div class="clearfix colelem" id="pu4561"><!-- group -->
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<iframe class="actAsDiv" width="513" height="320" src="http://www.youtube.com/embed/1-PRCBLqYT0?autoplay=0&loop=0&showinfo=0&theme=dark&color=red&controls=1&modestbranding=0&start=0&fs=1&iv_load_policy=1&wmode=transparent&rel=1" frameborder="0" allowfullscreen></iframe>
  
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<p>
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</div>
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If you are working on art and design as your main project, please join the art and design track. If you are integrating art and design into the core of your main project, please apply for the award by completing this page.
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    <div class="grpelem" id="u4563"><!-- custom html -->
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</p>
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<iframe class="actAsDiv" width="513" height="320" src="http://www.youtube.com/embed/xLkV1Lm19S8?autoplay=0&loop=0&showinfo=0&theme=dark&color=red&controls=1&modestbranding=0&start=0&fs=1&iv_load_policy=1&wmode=transparent&rel=1" frameborder="0" allowfullscreen></iframe>
  
 
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    <div class="clearfix colelem" id="u4182-7"><!-- content -->
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    <p>The result showed an exciting achievement on our fire retardant bacteria, in which fire retardant coating can protect the cotton from flame for up to 1 min. The carbon formation occurs in the charring process further proves our idea that fuels burned with phosphorus&#45;containg materia will release water and form carbon layer.</p>
 +
    <p>&nbsp;</p>
 +
    <p>Charring reaction: (HPO3)n + Cx(H2O)y → [C]x+ (HPO3)n × yH2O</p>
 +
    </div>
 +
    <a class="anchor_item colelem" id="house"></a>
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    <div class="clearfix colelem" id="pu3840"><!-- group -->
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    <div class="clearfix grpelem" id="u3840"><!-- group -->
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      <div class="clearfix grpelem" id="u3841"><!-- group -->
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      <img class="grpelem" id="u3842-4" alt="House" width="124" height="48" src="images/u3842-4.png"/><!-- rasterized frame -->
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      <div class="clearfix grpelem" id="u3843-4"><!-- content -->
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        <p>*</p>
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      </div>
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      </div>
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      <div class="clearfix grpelem" id="u3844-4"><!-- content -->
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      <p>*</p>
 +
      </div>
 +
    </div>
 +
    <div class="clearfix grpelem" id="u3845-9"><!-- content -->
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      <p id="u3845-2">As you know, when fire starts to burn on your house, it will soon become a horrible disaster. A special paint material, called fireproof paint, can slow down the spread of fire, or even stop it!&nbsp; We’re interested to know how our genetically modified bacteria could stop fire as fireproof paint. We painted the bacteria on the walls of wooden house, and see if it can really resist the fire.</p>
 +
      <p id="u3845-3">&nbsp;</p>
 +
      <p id="u3845-5">Design</p>
 +
      <p id="u3845-7">We applied laser cutter to build our house model in Mindgao’s Fablab. We designed the model in the size of 80x60x85 mm3 and made up of chipboards. The pieces of cutted boards were assembled to form the wooden house.</p>
 +
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 +
    <p id="u3854-2">Flame test</p>
 +
    <p id="u3854-4">Samples: wooden house (80x60x85 mm3 )</p>
 +
    <p id="u3854-6">Condition: Ignition with gas fire gun for 45 seconds</p>
 +
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      <p>House uncoated as control</p>
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      <p>House bio&#45;coated with fire retardant bacteria</p>
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    <p>Once again, the amazing result indicated that the house coated with our modified bacteria can resist fire for up to 45 seconds in our flame test. It clearly showed that the charring was formed on the coatings on the burning house. The data obviously demonstrated that we discovered and created a novel fire retardant bio&#45;material. Phosphorylated and high contents of nitrogen in a protein could be recognized as fire retardant bio&#45;materials. Our hypothesis had been successfully confirmed.</p>
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Revision as of 06:10, 16 September 2015

Design

Prototype

After finding, analyzing and testing with our novel fire retardant bio-material of phosphorylated SR protein, we would like to challenge the effectiveness of our fire retardant bio-coatings on real products. Fire retardant clothes and fire retardant paint attract increasingly interest in the market and industry. We applied our prototypes of fire retardant bacteria which produce phosphorylated SR proteins to mixing cotton and painting on a model house.

 

Fire retardant bacteria were made as described in the procedure of protein inducation and analysis as well as flame test.

↓ Recover bacteria carrying genes of SR and SRPK1 proteins from -80°C stock

↓ Culture at 37°C O/N

↓ Dilute 100X with fresh media and incubate for 2 hr till OD between 0.6~1.0

↓ Add 1mM IPTG to induce protein expression for 4 hr

↓ Soak cotton in the culture media containing bacteria

Or ↓ Centrifuge the bacteria and paint on the house

↓ Dry the bacteria completely in the oven at 100°C over 30 min

↓ Subjected to flame test with gas fire gun as indicated condition

Cotton

*

*

Samples: cotton (5X5 cm2)

Condition: Ignition till burning up (cotton control) or continues to the end (bio-coating).

Cotton uncoated as control

Cotton bio-coated with fire retardant bacteria

The result showed an exciting achievement on our fire retardant bacteria, in which fire retardant coating can protect the cotton from flame for up to 1 min. The carbon formation occurs in the charring process further proves our idea that fuels burned with phosphorus-containg materia will release water and form carbon layer.

 

Charring reaction: (HPO3)n + Cx(H2O)y → [C]x+ (HPO3)n × yH2O

House

*

*

As you know, when fire starts to burn on your house, it will soon become a horrible disaster. A special paint material, called fireproof paint, can slow down the spread of fire, or even stop it!  We’re interested to know how our genetically modified bacteria could stop fire as fireproof paint. We painted the bacteria on the walls of wooden house, and see if it can really resist the fire.

 

Design

We applied laser cutter to build our house model in Mindgao’s Fablab. We designed the model in the size of 80x60x85 mm3 and made up of chipboards. The pieces of cutted boards were assembled to form the wooden house.

Flame test

Samples: wooden house (80x60x85 mm3 )

Condition: Ignition with gas fire gun for 45 seconds

House uncoated as control

House bio-coated with fire retardant bacteria

Once again, the amazing result indicated that the house coated with our modified bacteria can resist fire for up to 45 seconds in our flame test. It clearly showed that the charring was formed on the coatings on the burning house. The data obviously demonstrated that we discovered and created a novel fire retardant bio-material. Phosphorylated and high contents of nitrogen in a protein could be recognized as fire retardant bio-materials. Our hypothesis had been successfully confirmed.