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Description
We planned to design a traffic light with E. coli cells which express genes for different fluorescent proteins. We are currently using the following devices:
Promoters
We chose the most suitable constitutive promoter for RFP production to act as a red traffic light (parts BBa_J231XX series). For GFP, we chose to use pBAD or pLac/ T7 promoters to control GFP expression by the inducers.
Riboswitches
We are using theophylline riboswitch and thiamine pyrophosphate (TPP) ribozyme as riboswitches. We are in the process of manipulating plasmid DNA in which GFP gene downstream of theophylline riboswitch (part BBa_K598010) is replaced by RFP gene to control expression of RFP gene. TPP ribozyme is used to suppress the expression of GFP gene that is placed downstream of the ribozyme by the addition of thiamine or TPP. (part BBa_K598011)