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| − | <!-- Section #2 -->
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| − | <section id="about" data-speed="10" data-type="background">
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| | | | |
| − | <h5 style="text-align:center;">
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| − | <a name="myAnchor" id="myAnchor"></a><br><br><br>Safety First!</h5> <br>
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| − | <P style="text-align:center;">
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| − | <font color="white">
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| − | Saving the world starts with saving ourselves. You cannot save the world unless you’re in good shape. We put safety on the top priority <br>
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| − | in any lab works. We have been informed accurately of the lab safety rules as well as bio-safety regulations at the<br>
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| − | initial stage of the project in order to understand hazards and safety procedures. Also we received safety training from our instructor <br>
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| − | before commencing our lab work. Even though our project is included Bio-Safety Level 1, we know very well that<br>
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| − | there are still many risks that may be escalated into serious disaster. Furthermore, even when handling those risks that may not pose serious<br>
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| − | threats to the experienced scientists, we as young learners should be extra careful.<br><br>
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| − | </font>
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| − | </p>
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| | | | |
| | | | |
| − | <h5 style="text-align:center;">
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| − | <font color="white">
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| − | The rule of thumb is:
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| − | </font>
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| − | </h5>
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| | | | |
| − | <div class="inner">
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| − | <font color="white">
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| − | <div class="caption2">
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| − | <h5>
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| − | <font color="white"> 1 </font>
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| − | </h5>
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| − | <p>
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| − | Start with reading Safety Questions for overview.
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| − | </p>
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| − | </div>
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| | | | |
| − | <div class="caption2">
| + | |
| − | <h5>
| + | <!-- Section #2 --> |
| − | <font color="white"> 2</font>
| + | <section id="about" data-speed="10" data-type="background"> |
| − | </h5>
| + | </section> |
| − | <p>
| + | |
| − | Follow Elan Vital Safety Rules all the time.
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </font>
| + | |
| − | </div> | + | |
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| − | <div class="inner">
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| − | <font color="white">
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| − | <div class="caption2">
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| − | <h5>
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| − | <font color="white"> 3 </font>
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| − | </h5>
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| − | <p>
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| − | Before entering the laboratory, read <br>
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| − | GMS General Safety Rules <br>
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| − | and GMS Extra Rules again and follow them
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| − | </p>
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| − | </div>
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| | | | |
| − | <div class="caption2">
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| − | <h5>
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| − | <font color="white"> 4 </font>
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| − | </h5>
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| − | <p>
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| − | If you are unsure of the rules or confused, <br>
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| − | ask lab assistant and/or <br>
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| − | instructor before doing anything.
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| − | </p>
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| − | </div>
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| − | </font>
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| − | </div>
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| − | <br>
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| | | | |
| − | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/b/b3/Scroll_arrow_top.PNG"></a>
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| − | <h6 style="text-align:center;">
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| − | <font color="white">
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| − | To The Top
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| − | </font>
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| − | </h6>
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| − | </section>
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| | | | |
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| | <!-- Section #3 --> | | <!-- Section #3 --> |
| | <section id="maintext" data-speed="10" data-type="background"> | | <section id="maintext" data-speed="10" data-type="background"> |
| | + | </section> |
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| − | <br><br>
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| − | <P style="text-align:center;">
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| − | <font color="black">
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| − | We have conducted our experiments at Gachon University. Thus, we have always observed safety rules of the lab. We had Safety Education & Training Session <br>
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| − | before starting our lab work. Also, when we conducted our experiments, Gachon University staff supervised our work.
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| − | </font>
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| − | </p>
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| − | <br><br>
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| | | | |
| − | <h5 style="text-align:center;">
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| − | <font color="black">
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| − | Gachon University General Lab Safety Rule
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| − | </font>
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| − | </h5>
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| − | <br><br>
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| − | <div class="inner2">
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| − | <font color="black">
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| − | <div class="caption3">
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| | | | |
| − | <h5>
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| − | <font color="black"> 1 </font>
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| − | </h5>
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| − | <p>
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| − | 200 mL LB prepared fresh,<br>
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| − | non-autoclaved
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| − | </p>
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| − | </div>
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| | | | |
| − | <div class="caption3">
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| − | <h5>
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| − | <font color="black"> 2 </font>
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| − | </h5>
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| − | <p>
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| − | 3 g agar
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| − | </p>
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| − | </div>
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| − |
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| − |
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| − | <div class="caption3">
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| − | <h5>
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| − | <font color="black"> 3 </font>
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| − | </h5>
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| − | <p>
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| − | Shake until all solids <br>
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| − | are dissolved
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| − | </p>
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| − | </div>
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| − |
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| − | <div class="caption3">
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| − | <h5>
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| − | <font color="black"> 4 </font>
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| − | </h5>
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| − | <p>
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| − | Autoclave for 20 min within 2 hr
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| − | </p>
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| − | </div>
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| − |
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| − | <div class="caption3">
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| − | <h5>
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| − | <font color="black"> 5 </font>
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| − | </h5>
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| − | <p>
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| − | Keep it cool until it reaches <br>
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| − | around 40-50 °C
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| − | </p>
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| − | </div>
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| − |
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| − | <div class="caption3">
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| − | <h5>
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| − | <font color="black"> 6 </font>
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| − | </h5>
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| − | <p>
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| − | Add 200 μL of 1000x chloramphenicol <br>
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| − | and gently stir it. Be careful not <br>
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| − | to shake the bottle too long/hard so that
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| − | bubbles are created.<br>
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| − | </p>
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| − | </div>
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| − |
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| − | <div class="caption3">
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| − | <h5>
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| − | <font color="black"> 7 </font>
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| − | </h5>
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| − | <p>
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| − | Pour into empty petri dishes <br>
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| − | just enough to cover the surface<br>
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| − | (~20 mL per plate).<br>
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| − | In case that bubbles are in <br>
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| − | the plate, heat the plate surface <br>
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| − | carefully with a burner only <br>
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| − | until the bubbles are <br>
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| − | burst but the solution is heated.
| |
| − | </p>
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| − | </div>
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| − |
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| − | <div class="caption3">
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| − | <h5>
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| − | <font color="black"> 8 </font>
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| − | </h5>
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| − | <p>
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| − | Leave the plates at room <br>
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| − | temperature around one hour <br>
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| − | until it is solidified.
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| − | </p>
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| − | </div>
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| − | </font>
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| − | </div>
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| − |
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| − | <div class="caption3">
| |
| − | <h5>
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| − | <font color="black"> 9 </font>
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| − | </h5>
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| − | <p>
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| − | Solidified plates should be <br>
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| − | turned upside down for a <br>
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| − | few hours at room temperature,<br>
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| − | then stored at 4°C.
| |
| − | </p>
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| − | </div>
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| − | </font>
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| − | </div>
| |
| − |
| |
| − | </section>
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| | | | |
| | | | |
| | <!-- Section #4 --> | | <!-- Section #4 --> |
| | <section id="maintext2" data-speed="10" data-type="background"> | | <section id="maintext2" data-speed="10" data-type="background"> |
| − | <br>
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| − |
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| − |
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| − |
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| − | <div class="inner3">
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| − | <font color="white">
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| − | <div class="caption4">
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| − |
| |
| − | <h5>
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| − | <font color="white"> 10 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Person who leaves the lab last must make sure that power is disconnected, flammable material is isolated, dangerous material is safely kept, water is disconnected, lab is cleaned and locked, and order of the lab must be double- checked.
| |
| − | </p>
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| − | </div>
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| − |
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| − | <div class="caption4">
| |
| − | <h5>
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| − | <font color="white"> 11 </font>
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| − | </h5>
| |
| − | <p>
| |
| − | Power wiring appliance must be properly installed to accommodate the experiments. Cables, and lines must be checked regularly to confirm that they are capable of handling the electrical load.
| |
| − | </p>
| |
| − | </div>
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| − |
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
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| − | <font color="white"> 12 </font>
| |
| − | </h5>
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| − | <p>
| |
| − | All equipment must be connected with the proper power Cable and must not be connected in a multiple power socket.
| |
| − | </p>
| |
| − | </div>
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| − |
| |
| − | <div class="caption3">
| |
| − | <h5>
| |
| − | <font color="white"> 13 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Autoclave for 20 min within 2 hr
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption3">
| |
| − | <h5>
| |
| − | <font color="white"> 14 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Keep it cool until it reaches <br>
| |
| − | around 40-50 °C
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption3">
| |
| − | <h5>
| |
| − | <font color="white"> 15 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Add 200 μL of 1000x chloramphenicol <br>
| |
| − | and gently stir it. Be careful not <br>
| |
| − | to shake the bottle too long/hard so that
| |
| − | bubbles are created.<br>
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
| |
| − | <font color="white"> 16 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Pour into empty petri dishes <br>
| |
| − | just enough to cover the surface<br>
| |
| − | (~20 mL per plate).<br>
| |
| − | In case that bubbles are in <br>
| |
| − | the plate, heat the plate surface <br>
| |
| − | carefully with a burner only <br>
| |
| − | until the bubbles are <br>
| |
| − | burst but the solution is heated.
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
| |
| − | <font color="white"> 17 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Leave the plates at room <br>
| |
| − | temperature around one hour <br>
| |
| − | until it is solidified.
| |
| − | </p>
| |
| − | </div>
| |
| − | </font>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
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| − | <font color="white"> 18 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | <font color="white">
| |
| − | Solidified plates should be <br>
| |
| − | turned upside down for a <br>
| |
| − | few hours at room temperature,<br>
| |
| − | then stored at 4°C.
| |
| − | </font>
| |
| − | </p>
| |
| − | </div>
| |
| − | </font>
| |
| − | </div>
| |
| | </section> | | </section> |
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| | <!-- Section #5 --> | | <!-- Section #5 --> |
| | <section id="maintext" data-speed="10" data-type="background"> | | <section id="maintext" data-speed="10" data-type="background"> |
| − | <br> | + | </section> |
| | | | |
| | | | |
| − | <div class="inner3">
| |
| − | <font color="black">
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| − | <div class="caption3">
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| | | | |
| − | <h5>
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| − | <font color="black"> 19 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Inflammable chemicals including acetone, oil, or gas must be kept in the place where ventilation is easy and access is infrequent.
| |
| − | </p>
| |
| − | </div>
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| | | | |
| − | <div class="caption3">
| |
| − | <h5>
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| − | <font color="black"> 20 </font>
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| − | </h5>
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| − | <p>
| |
| − | Importing and exporting of high-pressure gas container must be done using transportation equipment.
| |
| − | </p>
| |
| − | </div>
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| | | | |
| − |
| |
| − | <div class="caption3">
| |
| − | <h5>
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| − | <font color="black"> 21 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | When storing high-pressure gas containers, they must be safely and firmly fixed, isolated from flammable substances and inflammatory materials.
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption3">
| |
| − | <h5>
| |
| − | <font color="black"> 21 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Experiments using flammable, explosive, toxic, or volatile gases or vapors must be carried out in a hood.
| |
| − | </p>
| |
| − | </div>
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| − |
| |
| − | <div class="caption3">
| |
| − | <h5>
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| − | <font color="black"> 22 </font>
| |
| − | </h5>
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| − | <p>
| |
| − | Reagents container or cabinets for storing reagents must be kept in cool, well-ventilated places without direct sunlight, and far away from fire and heat sources.
| |
| − | </p>
| |
| − | </div>
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| − |
| |
| − | <div class="caption3">
| |
| − | <h5>
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| − | <font color="black"> 23 </font>
| |
| − | </h5>
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| − | <p>
| |
| − | Experimental drugs shall be stored in a way not to be shaken by outside shock, and so that its storage bottles do not fall
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
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| − | <font color="black"> 24 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Toxic materials shall be handled using protective films, or other safety tents to prevent damages from splatter, heating or explosion so as to ensure the safety of the experimenter.
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
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| − | <font color="black"> 25 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Containers for chemical wastes shall not be left in the hallway or on the stairs, and shall not be stored in the corners of the laboratory or other invisible places.
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption5">
| |
| − | <h5>
| |
| − | <font color="black"> 26 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | <font color="black">
| |
| − | When performing microbiological tests, gloves shall be worn, and after the experiment, the used glassware shall be
| |
| − | washed through sterilization, and disposable supplies and medium shall be disposed of separately from general garbage.
| |
| − | </font>
| |
| − | </p>
| |
| − | </div>
| |
| − | </font>
| |
| − | </div>
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| − |
| |
| − |
| |
| − |
| |
| − | </font>
| |
| − | </div>
| |
| − | </section>
| |
| | | | |
| | | | |
| | <!-- Section #6 --> | | <!-- Section #6 --> |
| | <section id="maintext2" data-speed="10" data-type="background"> | | <section id="maintext2" data-speed="10" data-type="background"> |
| − | <br>
| |
| − |
| |
| − |
| |
| − | <div class="inner3">
| |
| − | <font color="white">
| |
| − | <div class="caption4">
| |
| − |
| |
| − | <h5>
| |
| − | <font color="white"> 27 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Inflammable chemicals including acetone, oil, or gas must be kept in the place where ventilation is easy and access is infrequent.
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
| |
| − | <font color="white"> 28 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | Importing and exporting of high-pressure gas container must be done using transportation equipment.
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="caption4">
| |
| − | <h5>
| |
| − | <font color="white"> 29 </font>
| |
| − | </h5>
| |
| − | <p>
| |
| − | When storing high-pressure gas containers, they must be safely and firmly fixed, isolated from flammable substances and inflammatory materials.
| |
| − | </p>
| |
| − | </div>
| |
| − | </font>
| |
| − | </div>
| |
| − |
| |
| | </section> | | </section> |
| | | | |
