Difference between revisions of "Team:Tec-Chihuahua/Safety"

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<h2>Safety in iGEM</h2>
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    <title>Halcyon Days - An Exclusive Freebie for Codrops by Peter Finlan.</title>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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                <li><a href="index.html">Home</a></li>
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                <li><a href="project.html">Project</a></li>
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                <li><a href="notebook.html">NoteBook</a></li>
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                <li><a href="parts.html">Parts</a></li>
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                <li><a href="safety.html">Safety</a></li>
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                <li><a href="modeling.html">Modeling</a></li>
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                <li><a href="hpractices.html">Human Practices</a></li>
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        <header id="home">
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          <section class="hero safety-background" id="hero">
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                <div class=" ">
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                <div class="col-md-8 col-md-offset-2 text-center team-header">
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                    <h1 id="safety-title">Safety</h1>
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                </div>
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                <div class="col-md-7">
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                    <h1 id="safety-quote">Laboratory Safety, -particularly biosafety-, is an important issue of concern, because it represents the safe handling of pathogenic microorganisms, personal safety and security of microbial goods that can guarantee their use for clinical, research and epidemiological purposes.
 +
iGEM Tec-Chihuahua is especially commited with the safety area of the project. We  paid attention to all the details in the projects to assure our well being and that of others who may have access to the lab, such as students, profesors, cleaning personnel and visitors; also, the practices to maintain security in our laboratory provides more certainty for better experimental results.</h1>
 +
                </div>
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                <div class="col-md-5">
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                    <ul id="safety-menu">
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                        <li><a href="#training">Training</a></li>
 +
                        <li><a href="#labsafety">Lab Safety</a></li>
 +
                        <li><a href="#organisms">Organisms we use</a></li>
 +
                        <li><a>Forms</a></li>
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                    </ul>
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                </div>
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              </div>
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              <div class="row">
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        </header>
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        <section class="text-center">
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            <div class="container">
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                <h1 id="training" class="arrow">Training</h1>
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                <div class="row">
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                    <div class="col-md-12">
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                        <h2>Each member of the team took three online courses about Laboratory Biosafety and Biosecurity offered by the Public Health Agency of Canada (PHAC):</h2>
 +
                    </div>
 +
                    <div class="col-md-10 training-courses-left">
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                        <div class="col-md-10">
 +
                        <h1>General Safety for Containment Labs</h1>
 +
                        <p>Incorrect technical and human errors can threaten even the best measures implemented to protect the team. Therefore, what is essential to prevent acquired infections, incidents and accidents in the laboratory is a personal who knows and concerned about safety and how to recognize and combat the dangers of working in a laboratory.</p>
 +
                        </div>
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                        <div class="col-md-2">
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                        <img src="img/medal.png" >
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                    </div>
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                    </div>
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                    <div class="col-md-10 col-md-offset-2  training-courses-right">
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                        <div class="col-md-2">
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                            <img src="img/medal.png" >
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                        </div>
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                        <div class="col-md-10">
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                            <h1>Containment Level 2 Operational Practices</h1>
 +
                            <p>According to the WHO, our  laboratory is classified as a Basic Laboratory BSL2 (biosafety level 2); that means it is suitable for working with risk group 2: pathogens that can cause human or animal disease but are unlikely to cause a serious risk to laboratory personnel. The laboratory exposure can cause serious infection, but there are effective preventive and therapeutic measures and the risk of spread is limited.</p>
 +
                        </div>
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                    </div>
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                    <div class="col-md-8 training-courses-left">
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                        <div class="col-md-10">
 +
                            <h1>Personal Protective Equipment (PPE)</h1>
 +
                            <p>As students is very important to know the proper use of PPE, as it is a method of biological control risk that significantly reduces the risk to us and prevents the transport of organisms outside the containment laboratory by clothing. In this course we learn about the correct use of gown, gloves, and eye, face and feet protection.</p>
 +
                        </div>
 +
                        <div class="col-md-2">
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                            <img src="img/medal.png" >
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                        </div>
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                    </div>
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                    <div class="col-md-12 training-links">
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                        <h2>Here you can see the <a href="http://www.phac-aspc.gc.ca">PHAC web</a> and the <a target="_blank" href="/PHAC-certificates.pdf">certificates</a> that we recived.</h2>           
 +
                       
 +
                        <h2>Also, we received training in biological safety by ECOSERVICIOS DEL NORTE, a company that is in charge of the biohazard residues generated in the labs of our campus. Here you can see the <a target="_blank" href="/BIOHAZARD-COURSE-CERTIFICATES.pdf" >certificates</a> that we received for completing the course.</h2>
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                    </div>
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 +
                   
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                </div>
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            </div>
 +
        </section>
 +
        <section class="intro text-center clear-intro" id="intro">
 +
          <div class="container">
 +
            <h1 id="labsafety" class="arrow">Lab Safety</h1>
 +
            <h2 class="lab-safety-desc">All the experiments of our project were performed following the all the rules in order to make sure that our work is safe, specifically we follow the <a target="_blank" href="/lab-manual.pdf">Laboratory Manual</a> implemented in the Labs of our Campus.</h2>
 +
          </div>
 +
            <h1 id="organisms" class="arrow">Organisms we use</h1>
 +
            <h2>E coli</h2>
 +
            <p class="organisms-organism"  >This organism is listed by the American Biological Safety Association as part of the risk group level 2. It is pathogenic in humans and animals, but not in plants. Currently DH5α is most often used for routine cloning applications strain.
 +
</p>
 +
            <h2>Bos Taurus (oocytes)</h2>
 +
            <p class="organisms-organism">The most common and cheapest way to obtain oocytes is from slaughterhouse ovaries, so there is ample knowledge of the factors affecting the collection of ovaries. That said, the oocytes of slaughtered animals, represent a viable alternative for the production of embryos, wich can be used to basic research and/or practice of other reproductive technologies.
 +
It is important to acknowledge that the animals from which the cells of interest  are obtained, are treated with respect and are destined for human consumption.
 +
The lab work only reached the in vitro maturation of bovine oocytes, not fertilization. The technique to follow can be permormed in a variety of growing conditions depending on the environment in which the in vivo oocytes are. To mention an example, the good management of the incubator, the workspace and the sterile conditions where these are handled must be verified.
 +
                </p>
 +
            <h2>Nicotiana tabacum</h2>
 +
            <p class="organisms-organism">Transgenic plants expressing genes that confer tolerance to herbicides or resistance to insects is currently the subject of controversy in several parts of the world. The debate revolves around the safety that  these plants offer when they are used as food, as well as the long-term ecological consequences of their cultivation. In our case, the specimen that we will use, will not be used for this aim, but to evaluate a new technique of transformation, but still, a risk assessment is needed to verify that the inserted gene doesn´t have any harmful activity such as:
 +
</p>
 +
            <ul class="metabolits">
 +
                <li>Toxins</li>
 +
                <li>Cytokines</li>
 +
                <li>Hormones</li>
 +
                <li>Regulators of gene expression</li>
 +
                <li>Virulence factors or enhancers virulence</li>
 +
                <li>Oncogenic Sequences</li>
 +
                <li>Resistance to antibiotics</li>
 +
                <li>Allergens</li>
  
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
            </ul>
 
+
            <p class="organisms-organism">The use of this organism needs careful consideration and following of the rules, restrictions, and requirements for working with GMOs under the law of biosafety of genetically modified organisms issued by the Mexican Government on March 18, 2005.</p>
 
+
            <h1 id="forms" class="arrow">Forms</h1>
<h4>Safe Project Design</h4>
+
            <h3>For further analysis of our project, you can see here the iGEM forms that we completed</h3>
 
+
            <ul id="safety-forms" >
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
                <li><a href="https://2015.igem.org/Safety/About_Our_Lab?team_id=1852">About our Lab</a></li>
 
+
                <li><a href="https://2015.igem.org/Safety/About_Our_Project?team_id=1852">About our Project</a></li>
<ul>
+
                <li><a href="https://2015.igem.org/Safety/Final_Safety_Form?team_id=1852">Safety Form</a></li>
<li>Choosing a non-pathogenic chassis</li>
+
            </ul>
<li>Choosing parts that will not harm humans / animals / plants</li>
+
            <h2>Bibliography</h2>
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
            <p class="reference">World Health Organization (2004) Laboratory Biosafety Manual. [pdf] Recovered from <a href="http://www.who.int/csr/resources/publications/biosafety/en/Biosafety7.pdf"> http://www.who.int/csr/resources/publications/biosafety/en/Biosafety7.pdf</a> at 08/10/2015</p>
<li>Including an "induced lethality" or "kill-switch" device</li>
+
            <p class="reference" >Congreso de los Estados Unidos Mexicanos (2005) Ley de Bioseguridad de Organismos Genéticamente Modificados DOF-18-03-2005. Recovered from <a target="_blank" href="http://www.diputados.gob.mx/LeyesBiblio/pdf/LBOGM.pdf">http://www.diputados.gob.mx/LeyesBiblio/pdf/LBOGM.pdf</a> at  10/05/2015</p>
</ul>
+
          </section>
 
+
       
<h4>Safe Lab Work</h4>
+
        <footer class="text-center">
 
+
            <div class="col-md-8 col-md-offset-2">
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
                <div class="col-md-4">
 
+
                    <p>Address</p>
<h4>Safe Shipment</h4>
+
                    <p>Av. Heróico Colegio Militar 4700 Col. Nombre de Dios, Zip Code: 31300 </p>
 
+
                </div>
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
                <div class="col-md-4">
 
+
                    <p>Phone Number</p>
 
+
                    <p>+52 (614) 439 5000 (Ext. 3009)</p>
</div>
+
                </div>
</html>
+
                <div class="col-md-4">
 +
                    <p>Email</p>
 +
                    <p>igem_chih@outlook.com</p>
 +
                </div>
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Revision as of 20:08, 16 September 2015

Carbon carriers Halcyon Days - An Exclusive Freebie for Codrops by Peter Finlan.

Safety

Laboratory Safety, -particularly biosafety-, is an important issue of concern, because it represents the safe handling of pathogenic microorganisms, personal safety and security of microbial goods that can guarantee their use for clinical, research and epidemiological purposes. iGEM Tec-Chihuahua is especially commited with the safety area of the project. We paid attention to all the details in the projects to assure our well being and that of others who may have access to the lab, such as students, profesors, cleaning personnel and visitors; also, the practices to maintain security in our laboratory provides more certainty for better experimental results.

Training

Each member of the team took three online courses about Laboratory Biosafety and Biosecurity offered by the Public Health Agency of Canada (PHAC):

General Safety for Containment Labs

Incorrect technical and human errors can threaten even the best measures implemented to protect the team. Therefore, what is essential to prevent acquired infections, incidents and accidents in the laboratory is a personal who knows and concerned about safety and how to recognize and combat the dangers of working in a laboratory.

Containment Level 2 Operational Practices

According to the WHO, our laboratory is classified as a Basic Laboratory BSL2 (biosafety level 2); that means it is suitable for working with risk group 2: pathogens that can cause human or animal disease but are unlikely to cause a serious risk to laboratory personnel. The laboratory exposure can cause serious infection, but there are effective preventive and therapeutic measures and the risk of spread is limited.

Personal Protective Equipment (PPE)

As students is very important to know the proper use of PPE, as it is a method of biological control risk that significantly reduces the risk to us and prevents the transport of organisms outside the containment laboratory by clothing. In this course we learn about the correct use of gown, gloves, and eye, face and feet protection.

Lab Safety

All the experiments of our project were performed following the all the rules in order to make sure that our work is safe, specifically we follow the Laboratory Manual implemented in the Labs of our Campus.

Organisms we use

E coli

This organism is listed by the American Biological Safety Association as part of the risk group level 2. It is pathogenic in humans and animals, but not in plants. Currently DH5α is most often used for routine cloning applications strain.

Bos Taurus (oocytes)

The most common and cheapest way to obtain oocytes is from slaughterhouse ovaries, so there is ample knowledge of the factors affecting the collection of ovaries. That said, the oocytes of slaughtered animals, represent a viable alternative for the production of embryos, wich can be used to basic research and/or practice of other reproductive technologies. It is important to acknowledge that the animals from which the cells of interest are obtained, are treated with respect and are destined for human consumption. The lab work only reached the in vitro maturation of bovine oocytes, not fertilization. The technique to follow can be permormed in a variety of growing conditions depending on the environment in which the in vivo oocytes are. To mention an example, the good management of the incubator, the workspace and the sterile conditions where these are handled must be verified.

Nicotiana tabacum

Transgenic plants expressing genes that confer tolerance to herbicides or resistance to insects is currently the subject of controversy in several parts of the world. The debate revolves around the safety that these plants offer when they are used as food, as well as the long-term ecological consequences of their cultivation. In our case, the specimen that we will use, will not be used for this aim, but to evaluate a new technique of transformation, but still, a risk assessment is needed to verify that the inserted gene doesn´t have any harmful activity such as:

  • Toxins
  • Cytokines
  • Hormones
  • Regulators of gene expression
  • Virulence factors or enhancers virulence
  • Oncogenic Sequences
  • Resistance to antibiotics
  • Allergens

The use of this organism needs careful consideration and following of the rules, restrictions, and requirements for working with GMOs under the law of biosafety of genetically modified organisms issued by the Mexican Government on March 18, 2005.

Forms

For further analysis of our project, you can see here the iGEM forms that we completed

Bibliography

World Health Organization (2004) Laboratory Biosafety Manual. [pdf] Recovered from http://www.who.int/csr/resources/publications/biosafety/en/Biosafety7.pdf at 08/10/2015

Congreso de los Estados Unidos Mexicanos (2005) Ley de Bioseguridad de Organismos Genéticamente Modificados DOF-18-03-2005. Recovered from http://www.diputados.gob.mx/LeyesBiblio/pdf/LBOGM.pdf at 10/05/2015

Address

Av. Heróico Colegio Militar 4700 Col. Nombre de Dios, Zip Code: 31300

Phone Number

+52 (614) 439 5000 (Ext. 3009)

Email

igem_chih@outlook.com