Attributions

Project design

Our whole team met up once every week to share ideas on what project that we were going to carry out during the summer. We identified a problem that we thought should be improved and found a practical and biocompatible answer. The project that we have designed is to create nanowire amyloid formed from E.coli creating a more biocompatible and cheaper alternative to current materials. We were inspired by the pioneering work carried out by ... Hochschild and ... which provided up with .... The amyloid that we have produced has been made using Sup35NM and has additional cytochrome and heam which our bioscience team will carry out allowing it to conduct electricity. More on the description of our project can be found here .

Modelling

We wanted to find a way of visually representing the amyloid production of the E.coli bacteria that we have transformed in 3D. This was carried out using Matlab by James through reading research papers and using Brownian dynamics and the Monte Carlo method. Our model is available to view here .

Outreach

Finding out what the general public and members of parliament thought of our idea was carried out through questionnaires sent through mail and posted on social networks. In order to develop a wider understanding of how feasible our product is in the real world. A social networking team was created to allow us to communicate with other iGEM teams as well as the general public and political figures to gather their opinions and tell them more about our project. More information on outreach can be found here .

Wet lab

The wet lab work was naturally given to the bioscientists within the team who have carried out a vast range of protocols including but not exclusively AFM (Atomic Force Microscopy),Gibson Assembly, Ligation and PCR (Polymerase Chain Reaction). This was vital to the success of our project and was our main focus throughout the competition. More of our Protocols and experiments can be found here .

Writing

Throughout the course of iGEM the whole team have been carrying out research and reading papers related to our project to allow us all to contribute to writing up our final project. We have weekly meetings where we talk to one another about what has been done during the week which we record in our notebook which you can find here . We have designed and written posters, presentations and in depth explanations into our project that the whole team have helped produce.

Team

• Raneem AlJaghoub

  Raneem was part of the outreach team, developing the Policies and Practices part of our project. She interviewed international politicians and developed a questionnaire on synthetic biology. Raneem also contributed in the improvement of the wiki as well as constructing the parameters list for the modelling and designing the project. Raneem was a presenter at the Boston Jamboree.



• James Aston

  James developed the model of our system; he went the extra mile and rendered the equations into a code and afterwards into a 3D animation of our system. James also created the wiki skeleton and improved it during the competition. He collaborated the UNITN iGEM team by helping to create a model for their system. He also designed the project. James was a presenter at the Boston Jamboree.



• Anna Beltrami

  Anna was part of the wet lab team. In particular, she performed transformations, produced competent cells and helped capture pictures of the control samples with the Atomic Force Microscope. Anna constructed the organisational infrastructure of the team. She managed the social network channels and the overall communications with other teams, liasing collaborations. She helped review the wiki and she designed the project. Anna was a presenter at the Boston Jamboree.



• Moyra Chikomo

  Moyra was part of the outreach team, developing the Policies and Practices part of our project. She wrote post notes and helped in the creation of a questionnaire on synthetic biology sent to politicians and the general public. She designed and created a GUI (graphic used interface) for our projects as well as designing our banner.



• Richard Crane

  Richard was part of the wet lab team. In particular, he took part in most of the Lab work. His main contribution lies specifically in the imaging of the congo red and heme plates for the validation part of our project. He has made most agarose gels within the team.



• Rob Middlewick

  Rob was involved in lab work where he did mostly transformations as well as helping other members of the team with various protocol. He has played a key part in the scientific writing of our project, this included the project description and the description of our BioBricks.



• Katarina Pisani

  Kat being part of the main lab team was involved in all the wet lab aspects of our project. She has carried out ligations, PCR reactions and run diagnostic gels as well as much more. She has also presented at our outreach events and contributed to our final project design.



• Jonathan Pope

  Jonathan was also responsible for creating our posters and Envirowire logo. He has presented at our outreach events and has also assisted the lab team. He has designed our presentations and helped with the scientific and wiki writing. He has also held the main wet lab team in some protocol including carrying out diagnostic Congo red plating and transformations. Jonathan is also presenting at the Boston Jamboree.



• Natalie Sloan

  Natalie took part in designing and writing the wiki and creating the Kent iGEM logo. She is also part of the main wet lab team where she has carried out mini-preps, ligations and transformations as well as producing competent cells. Natalie has also helped design the project and contributed to scientific writing.



• Suparna Thakali

  Suparna contributed mainly to the wet lab team where she took part in carrying out Gibson assembly and ligation reactions. She contributed to the wiki page as well as project design and writing.



• Alice Tomlinson

  Alice was involved in organising the outreach aspect of our project such as gathering MPs opinion on synthetic biology and its possible applications in our society. She also conducted interviews and presented our project to other teams at our meet-ups. The majority of her time has been spent in the lab carrying out Gibson assembly and diagnostics tests.

Supervisors

Advisors

We would like to thank the following for their support: