Difference between revisions of "Team:elan vital korea/Project Overview"

 
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                 <h4 style="text-align:center;">
 
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                     PROJECT <br> -Project Overview-
 
                     PROJECT <br> -Project Overview-
 
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                    <font color="black">PROJECT OVERVIEW</font>
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Antibiotic-resistant bacteria pose a serious problem for global medical community. Detecting antibiotic resistance as quickly as possible is crucial for determination of the correct treatment for patients and for setting up quarantines to prevent spreading. We hypothesized that it is possible to use quorum sensing (QS) to devise a rapid way for cells to report the existence of antibiotic-resistant bacteria.
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the 1500s, when an unknown printer took a galley of type and scrambled it to make a type <br>
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specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, <br>
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remaining essentially unchanged. It was popularised in is simply dummy text of the printing and <br>
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text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a <br>
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Here, we developed a reporter cell that expresses GFP in the presence of the QS signaling molecule acyl homoserine lactone (AHL). Our test cells (which act as a simulation of antibiotic-resistant bacteria) express lactonase, which breaks down AHL. In our experimental system, test cells should signify their presence by breaking down AHL and preventing GFP expression in reporter cells. Therefore, our project serves as a proof of principle and we hope that our work will serve as a basis for developing similar, more sophisticated quorum sensing-based detection systems for antibiotic-resistant bacteria in the future.
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type book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining <br>
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essenti ally unchanged. It was popularised in the 1960 with the release of Letraset sheets <br>
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containing Lorem Ipsum passages, and more recently with desktop publishing is simply dummy text of the <br>
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printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since <br>
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the 1500s, when an unknown printer took a galley of type and scrambled it to make a type <br>
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specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, <br>
+
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remaining essentially unchanged. It was popularised in is simply dummy text of the printing and <br>
+
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typesetting industry. Lorem Ipsum has been the indust <br><br><br>
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text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a <br>
 
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type book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining <br>
 
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essenti ally unchanged. It was popularised in the 1960 with the release of Letraset sheets <br>
 
−
containing Lorem Ipsum passages, and more recently with desktop publishing is simply dummy text of the <br>
 
−
printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since <br>
 
−
the 1500s, when an unknown printer took a galley of type and scrambled it to make a type <br>
 
−
specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, <br>
 
−
remaining essentially unchanged. It was popularised in is simply dummy text of the printing and <br>
 
−
typesetting industry. Lorem Ipsum has been the indust <br><br><br>
 
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text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a <br>
 
−
type book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining <br>
 
−
essenti ally unchanged. It was popularised in the 1960 with the release of Letraset sheets <br>
 
−
containing Lorem Ipsum passages, and more recently with desktop publishing is simply dummy text of the <br>
 
−
printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since <br>
 
−
the 1500s, when an unknown printer took a galley of type and scrambled it to make a type <br>
 
−
specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, <br>
 
−
remaining essentially unchanged. It was popularised in is simply dummy text of the printing and <br>
 
−
typesetting industry. Lorem Ipsum has been the indust <br><br><br>
 
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Latest revision as of 17:40, 17 September 2015








PROJECT
-Project Overview-









PROJECT OVERVIEW


Antibiotic-resistant bacteria pose a serious problem for global medical community. Detecting antibiotic resistance as quickly as possible is crucial for determination of the correct treatment for patients and for setting up quarantines to prevent spreading. We hypothesized that it is possible to use quorum sensing (QS) to devise a rapid way for cells to report the existence of antibiotic-resistant bacteria.



Here, we developed a reporter cell that expresses GFP in the presence of the QS signaling molecule acyl homoserine lactone (AHL). Our test cells (which act as a simulation of antibiotic-resistant bacteria) express lactonase, which breaks down AHL. In our experimental system, test cells should signify their presence by breaking down AHL and preventing GFP expression in reporter cells. Therefore, our project serves as a proof of principle and we hope that our work will serve as a basis for developing similar, more sophisticated quorum sensing-based detection systems for antibiotic-resistant bacteria in the future.



To The Top