|
|
| (138 intermediate revisions by the same user not shown) |
| Line 59: |
Line 59: |
| | } | | } |
| | #about { | | #about { |
| − | background: url(https://static.igem.org/mediawiki/2015/8/80/Experimentalbg.png) 50% 0 no-repeat fixed; | + | background: url(https://static.igem.org/mediawiki/2015/9/9c/Newlightbg.jpg) 50% 0 no-repeat fixed; |
| | min-height:1000px; | | min-height:1000px; |
| | height: 1000px; | | height: 1000px; |
| Line 69: |
Line 69: |
| | } | | } |
| | #maintext { | | #maintext { |
| − | background: url(https://static.igem.org/mediawiki/2015/1/1a/ExperimentalBG3.PNG) 50% 0 no-repeat fixed; | + | background: url(https://static.igem.org/mediawiki/2015/9/9c/Newlightbg.jpg) 50% 0 no-repeat fixed; |
| | height: 1000px; | | height: 1000px; |
| | min-height:1000px; | | min-height:1000px; |
| Line 80: |
Line 80: |
| | | | |
| | #maintext2 { | | #maintext2 { |
| − | background: url(https://static.igem.org/mediawiki/2015/b/bb/ExperimentalBG2.PNG) 50% 0 no-repeat fixed; | + | background: url(https://static.igem.org/mediawiki/2015/9/9c/Newlightbg.jpg) 50% 0 no-repeat fixed; |
| | height: 1000px; | | height: 1000px; |
| | min-height:1000px; | | min-height:1000px; |
| Line 90: |
Line 90: |
| | } | | } |
| | | | |
| − | #maintext3 {
| |
| − | background: url(https://static.igem.org/mediawiki/2015/1/1a/ExperimentalBG3.PNG) 50% 0 no-repeat fixed;
| |
| − | height: 1000px;
| |
| − | min-height:1000px;
| |
| − | margin: 0 auto;
| |
| − | width: 100%;
| |
| − | max-width: 1920px;
| |
| − | position: relative;
| |
| − | box-shadow: 0 0 50px rgba(0,0,0,0.8);
| |
| − | }
| |
| | | | |
| − | #maintext4 {
| |
| − | background: url(https://static.igem.org/mediawiki/2015/b/bb/ExperimentalBG2.PNG) 50% 0 no-repeat fixed;
| |
| − | height: 1000px;
| |
| − | min-height:1000px;
| |
| − | margin: 0 auto;
| |
| − | width: 100%;
| |
| − | max-width: 1920px;
| |
| − | position: relative;
| |
| − | box-shadow: 0 0 50px rgba(0,0,0,0.8);
| |
| − | }
| |
| − |
| |
| − | #maintext5 {
| |
| − | background: url(https://static.igem.org/mediawiki/2015/1/1a/ExperimentalBG3.PNG) 50% 0 no-repeat fixed;
| |
| − | height: 1000px;
| |
| − | min-height:1000px;
| |
| − | margin: 0 auto;
| |
| − | width: 100%;
| |
| − | max-width: 1920px;
| |
| − | position: relative;
| |
| − | box-shadow: 0 0 50px rgba(0,0,0,0.8);
| |
| − | }
| |
| | | | |
| | | | |
| Line 135: |
Line 104: |
| | | | |
| | #maintext2 article { background: url("#") no-repeat scroll center top transparent; height: 458px; position: absolute; text-indent: -9999px; top: 291px; width: 100%; } | | #maintext2 article { background: url("#") no-repeat scroll center top transparent; height: 458px; position: absolute; text-indent: -9999px; top: 291px; width: 100%; } |
| − |
| |
| − | #maintext3 article { background: url("#") no-repeat scroll center top transparent; height: 458px; position: absolute; text-indent: -9999px; top: 291px; width: 100%; }
| |
| | | | |
| | | | |
| Line 184: |
Line 151: |
| | | | |
| | h4 { | | h4 { |
| − | color:white; | + | color:black; |
| | font-size: 24px; | | font-size: 24px; |
| | letter-spacing:1px; | | letter-spacing:1px; |
| Line 199: |
Line 166: |
| | | | |
| | p { | | p { |
| − | font-size:16px; | + | font-size:18px; |
| − | line-height:180%; | + | line-height:160%; |
| | } | | } |
| | | | |
| Line 237: |
Line 204: |
| | | | |
| | | | |
| | + | .inner { |
| | + | width: 50%; |
| | + | height: auto; |
| | + | margin-left:25%; |
| | + | } |
| | + | |
| | </style> | | </style> |
| | | | |
| Line 244: |
Line 217: |
| | <!-- Section #1 --> | | <!-- Section #1 --> |
| | <section id="home" data-speed="10" data-type="background"> | | <section id="home" data-speed="10" data-type="background"> |
| − | <a name="top" id="top"></a> | + | <a name="top" id="top"></a> |
| − | <a href="#"><img class="igem" src="https://static.igem.org/mediawiki/2015/7/70/IGEM_Logo.gif"></a> | + | |
| − | <br>
| + | <img class="igem" src="https://static.igem.org/mediawiki/2015/7/70/IGEM_Logo.gif"> |
| − | <br>
| + | </a> |
| − | <br>
| + | <br><br><br><br><br><br><br> |
| − | <br>
| + | |
| − | <br>
| + | <a href="https://2015.igem.org/Team: Elan_Vital_Korea"> |
| − | <br>
| + | <img class="displayed" src="https://static.igem.org/mediawiki/2015/d/d5/ElanVital_Logo.png"> |
| − | <br>
| + | </a> |
| − | <div class="center;"> | + | |
| − | <a href="https://2015.igem.org/Team: elan_vital_korea"> | + | |
| − | <img class="displayed" src="https://static.igem.org/mediawiki/2015/d/d5/ElanVital_Logo.png"> | + | |
| − | </a>
| + | |
| | <h4 style="text-align:center;"> | | <h4 style="text-align:center;"> |
| | + | <font color="white"> |
| | PROJECT <br> -Project Overview- | | PROJECT <br> -Project Overview- |
| | </h4> | | </h4> |
| − | <a href="#myAnchor" rel="" id="anchor1" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/d/d2/Scroll_arrow.png"></a> | + | </font> |
| | + | <a href="#myAnchor" rel="" id="anchor1" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/d/d2/Scroll_arrow.png"></a> |
| | </div> | | </div> |
| | </section> | | </section> |
| | | | |
| − | <!-- Section #2 -->
| |
| − | <section id="about" data-speed="10" data-type="background">
| |
| | | | |
| − | <h5 style="text-align:center;">
| |
| − | <a name="myAnchor" id="myAnchor"></a><br><br><br><br><br><br><br><br><br><font color="white">PROJECT OVERVIEW</font></h5> <br><br><br>
| |
| − | <P style="text-align:center;">
| |
| − | <font color="white">
| |
| − | Bacteria acquiring resistance to antibiotics pose serious health problem globally. Following last year’s example, <br>
| |
| − | the project of Elan Vital Korea for this year also is related to MRSA. This year, however, we have focused <br>
| |
| − | on early detection of MRSA infection using quorum sensing. Below, we have briefly described the health threats <br>
| |
| − | caused by MRSA, and have explained the quorum sensing method. Then, we have proceeded to the description <br>
| |
| − | of how we designed and implemented our experiments, and what results we have obtained. Finally, we have briefly <br>
| |
| − | outlined the implication of our results and future plans. <br><br><br>
| |
| | | | |
| − | </font>
| |
| − | </p>
| |
| | | | |
| − | <br>
| |
| − | <br>
| |
| − | <br><br><br><br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <br>
| |
| − | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/b/b3/Scroll_arrow_top.PNG"></a>
| |
| − | <h6 style="text-align:center;">
| |
| − | <font color="white">
| |
| − | To The Top
| |
| − | </font>
| |
| − | </h6>
| |
| | | | |
| | | | |
| − | </section>
| + | <!-- Section #2 --> |
| − | | + | <section id="about" data-speed="10" data-type="background"> |
| − | | + | <h5 style="text-align:center;"> |
| − | <!-- Section #3 --> | + | <a name="myAnchor" id="myAnchor"></a> |
| − | <section id="maintext" data-speed="10" data-type="background"> | + | <br><br><br><br><br><br><br><br> |
| − | <br><br><br> | + | <font color="black">PROJECT OVERVIEW</font> |
| − | | + | </h5> |
| − | | + | <br><br> |
| − | <font color="black"> | + | <div class="inner"> |
| − | <h5 style="text-align:center;"> | + | <P style="text-align: left;"> |
| − | Threats of Antibiotics-Resistant Bacteria
| + | <font color="black"> |
| − | </h5> | + | Antibiotic-resistant bacteria pose a serious problem for global medical community. Detecting antibiotic resistance as quickly as possible is crucial for determination of the correct treatment for patients and for setting up quarantines to prevent spreading. We hypothesized that it is possible to use quorum sensing (QS) to devise a rapid way for cells to report the existence of antibiotic-resistant bacteria. |
| − | <br><br><br> | + | </font> |
| − | <P style="text-align:center;"> | + | </p> |
| − | Infection by antibiotic-resistant bacteria is a serious health threat worldwide including Korea and <br>
| + | </div> |
| − | the United States of America. It is a serious threat primarily because, as the name suggests, <br>
| + | |
| − | bacteria have evolutionarily developed a resistance to antibiotics. It means, first of all, drugs don’t work.<br> | + | |
| − | Furthermore, the spread of the antibiotic-resistant bacteria makes it more difficult to <br>
| + | |
| − | control or contain the spread of the infectious disease, because it undermines the effectiveness of treatment.<br>
| + | |
| − | And, it substantially increases the cost of healthcare, and the burden to society because it prolongs <br>
| + | |
| − | the treatment period and increases the likelihood of death. WHO declared that it “threatens the achievements of <br>
| + | |
| − | modern medicine” (Antimicrobial Resistance: Global Report on Surveillance 2014, WHO, 2014). <br>
| + | |
| − | Antimicrobial resistance already causes 700,000 deaths every year, which number is expected to 10 million annually<br>
| + | |
| − | by 2050 (An international legal framework to address antimicrobial resistance, WHO, 2015).
| + | |
| − | </p> | + | |
| − | </font> | + | |
| − | <br><br> | + | |
| − | <img class="displayed" src="https://static.igem.org/mediawiki/2015/7/74/Hr_black.jpg" width="80px" height="2px">
| + | |
| | <br><br> | | <br><br> |
| | + | </h6> |
| | | | |
| − | <P style="text-align:center;"> | + | <div class="inner"> |
| − | <font color="black"> | + | <P style="text-align: left;"> |
| − | What makes the problem more pressing is that the data isbased on the reports of clinical samples from <br>
| + | <font color="black"> |
| − | laboratories, “predominantly in hospital settings” (Antimicrobial Resistance: Global Report on <br>
| + | Here, we developed a reporter cell that expresses GFP in the presence of the QS signaling molecule acyl homoserine lactone (AHL). Our test cells (which act as a simulation of antibiotic-resistant bacteria) express lactonase, which breaks down AHL. In our experimental system, test cells should signify their presence by breaking down AHL and preventing GFP expression in reporter cells. Therefore, our project serves as a proof of principle and we hope that our work will serve as a basis for developing similar, more sophisticated quorum sensing-based detection systems for antibiotic-resistant bacteria in the future. |
| − | Surveillance 2014, WHO, 2014, p. 70), which means community-acquired (compared to health-care associated)<br>
| + | |
| − | infections and uncomplicated infections are underrepresented.
| + | |
| − | </font>
| + | |
| | </p> | | </p> |
| − | | + | </div> |
| − | <br><br> | + | </font> |
| | + | <br><br> |
| | | | |
| | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/5/5b/Scroll_arrow_top_Black.png"></a> | | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/5/5b/Scroll_arrow_top_Black.png"></a> |
| Line 341: |
Line 271: |
| | To The Top | | To The Top |
| | </font> </h6> | | </font> </h6> |
| | + | |
| | + | </section> |
| | | | |
| − | </section>
| |
| | | | |
| | | | |
| − | <!-- Section #4 -->
| |
| − | <section id="maintext2" data-speed="10" data-type="background">
| |
| − | <font color="white">
| |
| − | <h5 style="text-align:center;">
| |
| − | Existing Methods Used for Detection
| |
| − | </h5>
| |
| − | <br><br><br>
| |
| − |
| |
| − | <P style="text-align:center;">
| |
| − | <br><br>
| |
| − | CDC’s efforts at outsmarting the antibiotic resistance focuses on 4 core actions: detect, respond, prevent <br>
| |
| − | and discover. The project is called AR Initiative (Detect and Protect Against Antibiotic <br>
| |
| − | Resistance Initiative), which is an integral part of the CDC strategy to target investment aimed at AR. <br>
| |
| − | Among the AR initiative, detection is the first step that impacts the whole controlling process. <br>
| |
| − | Detecting antibiotic resistance quickly and effectively is crucial for determination of the treatment methods<br>
| |
| − | for different patients as well as for quarantines to prevent it from becoming epidemic. <br>
| |
| − | Currently, several methods are used for the detection of the antibiotic resistance. Most common and traditional <br>
| |
| − | method is using growth inhibition assays performed in broth or by agar disc diffusion. <br>
| |
| − | For clinically critical bacteria, diagnostic laboratories perform phenotypic-based analyses using standardized <br>
| |
| − | susceptibility testing methods, usually in accordance with the guidelines published by the Clinical <br>
| |
| − | and Laboratory Standards Institute. <br><br>
| |
| − | </font>
| |
| − | </p>
| |
| − |
| |
| − | <br><br>
| |
| − | <img class="displayed" src="https://static.igem.org/mediawiki/2015/6/6e/Hr_white.jpg" width="80px" height="2px">
| |
| − | <br><br>
| |
| − |
| |
| − |
| |
| − | <h5 style="text-align:center;">
| |
| − | <font color="white">
| |
| − | Lorem ipsum dolor sit amet
| |
| − | </font>
| |
| − | </h5>
| |
| − | <br><br>
| |
| − |
| |
| − |
| |
| − | <P style="text-align:center;">
| |
| − | <font color="white">
| |
| − | Bacteria acquiring resistance to antibiotics pose serious health problem globally. Following last year’s example, <br>
| |
| − | Using the culture-based approach, it can take 1—2 days to produce results for fast-growing bacteria such as <br>
| |
| − | Escherichia coli orSalmonella, but several weeks for slow-growing bacteria such as Mycobacterium tuberculosis. <br>
| |
| − | Moreover, culturing only works for a small fraction of microbes; although most pathogens can be cultured <br>
| |
| − | relatively easily thanks to years of accumulated experimental experiences, the vast majority of microbes cannot <br>
| |
| − | grow outside their host environment, including pathogens such as Chlamydia orTrypanosomes. <br><br>
| |
| − |
| |
| − | LUsing newer molecular detection techniques for antibiotic resistance such as quantitative PCR (qPCR) or <br>
| |
| − | microarrays, we can determine the presence of specific resistance genes within hours, and we obtain improved <br>
| |
| − | diagnosis results. However, these culture-independent approaches target well-known and well-studied<br>
| |
| − | pathogens or resistance-causing genes only, and cannot be easily used for broader spectrum screening. <br><br><br>
| |
| − | </font>
| |
| − | </p>
| |
| − |
| |
| − | <br>
| |
| − |
| |
| − | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/b/b3/Scroll_arrow_top.PNG"></a>
| |
| − | <h6 style="text-align:center;"> <font color="white">
| |
| − | To The Top
| |
| − | </font> </h6>
| |
| − | </section>
| |
| − |
| |
| − |
| |
| − | <!-- Section #5 -->
| |
| − | <section id="maintext3" data-speed="10" data-type="background">
| |
| − | <P style="text-align:center;">
| |
| − | <font color="black">
| |
| − | <br>
| |
| − | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec lacinia erat ex, ut dignissim orci luctus <br>
| |
| − | vitae. Nunc ante sapien, tempus vel commodo at, placerat in enim. Quisque elementum mauris sit amet <br>
| |
| − | tellus varius, ut hendrerit diam porttitor. Nunc feugiat tellus sed ipsum tristique, tristique porta lorem <br>
| |
| − | posuere. Quisque cursus egestas risus, ut ultricies leo ornare molestie. Vivamus pellentesque enim vel<br>
| |
| − | turpis cursus feugiat. Proin semper, ex. <br><br>
| |
| − | </font>
| |
| − | </p>
| |
| − |
| |
| − | <br><br>
| |
| − | <img class="displayed" src="https://static.igem.org/mediawiki/2015/6/6e/Hr_black.jpg" width="80px" height="2px">
| |
| − | <br><br>
| |
| − |
| |
| − |
| |
| − | <h5 style="text-align:center;">
| |
| − | <font color="black">
| |
| − | Existing Methods Used for Detection
| |
| − | </font>
| |
| − | </h5>
| |
| − | <br><br>
| |
| − |
| |
| − |
| |
| − | <P style="text-align:center;">
| |
| − | <font color="black">
| |
| − | CDC’s efforts at outsmarting the antibiotic resistance focuses on 4 core actions: detect, respond, prevent <br>
| |
| − | and discover. The project is called AR Initiative (Detect and Protect Against Antibiotic <br>
| |
| − | Resistance Initiative), which is an integral part of the CDC strategy to target investment aimed at AR. <br>
| |
| − | Among the AR initiative, detection is the first step that impacts the whole controlling process. <br>
| |
| − | Detecting antibiotic resistance quickly and effectively is crucial for determination of the treatment methods<br>
| |
| − | for different patients as well as for quarantines to prevent it from becoming epidemic. <br>
| |
| − | Currently, several methods are used for the detection of the antibiotic resistance. Most common and traditional <br>
| |
| − | method is using growth inhibition assays performed in broth or by agar disc diffusion. <br>
| |
| − | For clinically critical bacteria, diagnostic laboratories perform phenotypic-based analyses using standardized <br>
| |
| − | susceptibility testing methods, usually in accordance with the guidelines published by the Clinical <br>
| |
| − | and Laboratory Standards Institute.
| |
| − | <br><br><br>
| |
| − | </font>
| |
| − | </p>
| |
| − |
| |
| − | <br>
| |
| − |
| |
| − | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/5/5b/Scroll_arrow_top_Black.png"></a>
| |
| − | <h6 style="text-align:center;"> <font color="black">
| |
| − | To The Top
| |
| − | </font> </h6>
| |
| − | </section>
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − | <!-- Section #6 -->
| |
| − | <section id="maintext4" data-speed="10" data-type="background">
| |
| − | <P style="text-align:center;">
| |
| − | <font color="white">
| |
| − | <br><br>
| |
| − | Using the culture-based approach, it can take 1—2 days to produce results for fast-growing bacteria such as <br>
| |
| − | Escherichia coli orSalmonella, but several weeks for slow-growing bacteria such as Mycobacterium tuberculosis. <br>
| |
| − | Moreover, culturing only works for a small fraction of microbes; although most pathogens can be cultured <br>
| |
| − | relatively easily thanks to years of accumulated experimental experiences, the vast majority of microbes cannot <br>
| |
| − | grow outside their host environment, including pathogens such as Chlamydia orTrypanosomes. <br><br>
| |
| − |
| |
| − | LUsing newer molecular detection techniques for antibiotic resistance such as quantitative PCR (qPCR) or <br>
| |
| − | microarrays, we can determine the presence of specific resistance genes within hours, and we obtain improved <br>
| |
| − | diagnosis results. However, these culture-independent approaches target well-known and well-studied<br>
| |
| − | pathogens or resistance-causing genes only, and cannot be easily used for broader spectrum screening. <br><br>
| |
| − |
| |
| − | CDC dramatically innovated the detection process by adopting the Advanced Molecular Detection (AMD), <br>
| |
| − | which combines the latest pathogen identification technologies with bioinformatics and <br>
| |
| − | advanced epidemiology to more effectively understand, prevent and control infectious diseases. Using those <br>
| |
| − | technologies, it is possible to rapidly look for a microbe's match among thousands of reference samples<br>
| |
| − | in the microbe library. If no match is found, the whole genomic sequence of the microbe's <br>
| |
| − | DNA code can be taken, then quickly analyzed using disease detective works and bioinformatics to answer <br>
| |
| − | critical disease-response questions. However, this new method, while it sounds very interesting,<br>
| |
| − | is not to be completed until 2020, and still requires incubation, as well as being expensive. <br>
| |
| − | </font>
| |
| − | </p>
| |
| − |
| |
| − | <br><br>
| |
| − | <img class="displayed" src="https://static.igem.org/mediawiki/2015/6/6e/Hr_white.jpg" width="80px" height="2px">
| |
| − | <br><br>
| |
| − |
| |
| − | <br>
| |
| − |
| |
| − | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/b/b3/Scroll_arrow_top.PNG"></a>
| |
| − | <h6 style="text-align:center;"> <font color="white">
| |
| − | To The Top
| |
| − | </font> </h6>
| |
| − | </section>
| |
| − |
| |
| − |
| |
| − | <!-- Section #7 -->
| |
| − | <section id="maintext5" data-speed="10" data-type="background">
| |
| − | <P style="text-align:center;">
| |
| − | <font color="black">
| |
| − | <br>
| |
| − | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer luctus arcu in augue viverra egestas. <br>
| |
| − | Morbi volutpat maximus dolor, non lacinia augue laoreet sit amet. Maecenas sed nulla diam. <br>
| |
| − | Integer imperdiet vulputate justo, tristique dictum purus tempus at. Etiam sit amet turpis urna. Proin nec <br>
| |
| − | nulla at libero euismod accumsan. Sed pulvinar velit eu purus condimentum rutrum ac in arcu. Nam <br>
| |
| − | fringilla nec dui at scelerisque. Nam lacus est, pretium eu nisi eget, congue vehicula diam. Curabitur pell<br>
| |
| − | entesque dapibus sollicitudin. Vestibulum nec eros eget nibh aliquam pharetra in sit amet diam. <br>
| |
| − | Aenean blandit neque non orci viverra iaculis. Donec ligula quam, ultrices at. <br><br>
| |
| − |
| |
| − | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec at dui mi. Vestibulum purus elit, lacinia <br>
| |
| − | vitae elit nec, tempor rutrum purus. Duis ac tempus sem, eu sodales justo. Cras tristique <br>
| |
| − | lobortis efficitur. Donec sed turpis non ipsum sodales viverra. Proin vehicula quam in nisl laoreet finibus.<br>
| |
| − | Phasellus a vehicula justo, eu hendrerit tortor. Pellentesque faucibus, ex vel dictum tincidunt,<br>
| |
| − | lorem sem. <br>
| |
| − | </font>
| |
| − | </p>
| |
| − |
| |
| − | <br><br>
| |
| − | <img class="displayed" src="https://static.igem.org/mediawiki/2015/6/6e/Hr_black.jpg" width="80px" height="2px">
| |
| − | <br><br>
| |
| − |
| |
| − |
| |
| − | <h5 style="text-align:center;">
| |
| − | <font color="black">
| |
| − | Lorem ipsum dolor sit amet
| |
| − | </font>
| |
| − | </h5>
| |
| − |
| |
| − |
| |
| − | <P style="text-align:center;">
| |
| − | <font color="black">
| |
| − | <br>
| |
| − | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam rhoncus imperdiet eros, et sollicitudin tortor <br> egestas id. Aliquam vel sapien lacus. Etiam in est a orci dignissim semper a a nisl. Pellentesque<br>
| |
| − | ullamcorper libero sit amet accumsan vestibulum. Sed enim augue, placerat sed metus quis, posuere interdum lectus.<br>
| |
| − | Suspendisse ipsum orci, condimentum eu lorem sem. <br><br>
| |
| − | Lorem ipsum dolor sit amet, consectetur adipiscing elit. Duis eu mi blandit, tincidunt felis at, molestie augue. <br>
| |
| − | Aenean ac pharetra massa. Sed commodo tincidunt luctus. Nulla laoreet erat rhoncus, venenatis nunc vel,<br>
| |
| − | fermentum nunc. Curabitur rutrum orci ut neque ornare, et vehicula massa viverra. Ut congue tellus vel interdum mollis. <br>
| |
| − | Phasellus sed rutrum sapien. Cras hendrerit non neque ut posuere. <br>
| |
| − |
| |
| − | </font>
| |
| − | </p>
| |
| − |
| |
| − | <br>
| |
| − | <br>
| |
| − |
| |
| − | <a href="#top" rel="" id="top" class="anchorLink"><img class="displayed" src="https://static.igem.org/mediawiki/2015/5/5b/Scroll_arrow_top_Black.png"></a>
| |
| − | <h6 style="text-align:center;"> <font color="black">
| |
| − | To The Top
| |
| − | </font> </h6>
| |
| − |
| |
| − | </section>
| |
| | | | |
| | | | |
