Difference between revisions of "Team:elan vital korea/Project Overview"

 
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                     PROJECT <br> -Project Overview-
 
                     PROJECT <br> -Project Overview-
 
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Bacteria acquiring resistance to antibiotics pose serious health problem globally. Following last year’s example, <br>
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the project of Elan Vital Korea for this year also is related to MRSA.  This year, however, we have focused <br>
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Antibiotic-resistant bacteria pose a serious problem for global medical community. Detecting antibiotic resistance as quickly as possible is crucial for determination of the correct treatment for patients and for setting up quarantines to prevent spreading. We hypothesized that it is possible to use quorum sensing (QS) to devise a rapid way for cells to report the existence of antibiotic-resistant bacteria.
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on early detection of MRSA infection using quorum sensing.  Below, we have briefly described the health threats <br>
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caused by MRSA, and have explained the quorum sensing method.  Then, we have proceeded to the description <br>
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of how we designed and implemented our experiments, and what results we have obtained. Finally, we have briefly <br>
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outlined the implication of our results and future plans. <br><br><br>
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Here, we developed a reporter cell that expresses GFP in the presence of the QS signaling molecule acyl homoserine lactone (AHL). Our test cells (which act as a simulation of antibiotic-resistant bacteria) express lactonase, which breaks down AHL. In our experimental system, test cells should signify their presence by breaking down AHL and preventing GFP expression in reporter cells. Therefore, our project serves as a proof of principle and we hope that our work will serve as a basis for developing similar, more sophisticated quorum sensing-based detection systems for antibiotic-resistant bacteria in the future.
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Latest revision as of 17:40, 17 September 2015








PROJECT
-Project Overview-









PROJECT OVERVIEW


Antibiotic-resistant bacteria pose a serious problem for global medical community. Detecting antibiotic resistance as quickly as possible is crucial for determination of the correct treatment for patients and for setting up quarantines to prevent spreading. We hypothesized that it is possible to use quorum sensing (QS) to devise a rapid way for cells to report the existence of antibiotic-resistant bacteria.



Here, we developed a reporter cell that expresses GFP in the presence of the QS signaling molecule acyl homoserine lactone (AHL). Our test cells (which act as a simulation of antibiotic-resistant bacteria) express lactonase, which breaks down AHL. In our experimental system, test cells should signify their presence by breaking down AHL and preventing GFP expression in reporter cells. Therefore, our project serves as a proof of principle and we hope that our work will serve as a basis for developing similar, more sophisticated quorum sensing-based detection systems for antibiotic-resistant bacteria in the future.



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