Difference between revisions of "Team:Brasil-USP/Parts/Listofparts"

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<table style="width:100%" border="1">
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  <tr>
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    <td><b>Registry Code</b></td>
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    <td><b>Name</b></td>
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    <td><b>Role</b></td>
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    <td><b>Description</b></td>
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    <td><b>Length (bp)</b></td>
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  </tr>
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  <tr>
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    <td>BBa_K1819003</td>
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    <td>RoxA</td>
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    <td>Protein coding</td>
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    <td>Rubber oxygenase A (Rox A) from <i>Xanthomonas sp.</i> strain 35Y- catalyzes oxidative C-C cleavage of
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  poly(cis-1,4-isoprene)</td>
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    <td>1954 (bp)</td>
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  </tr>
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  <tr>
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    <td>BBa_K1819001</td>
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    <td>Lcp</td>
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    <td>Role</td>
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    <td>Latex clearing protein (Lcp) from <i>Streptomyces sp.</i> strain K30
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  - catalyzes rubber cleavage at the
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  cis double bonds</td>
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    <td>1138</td>
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  </tr>
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  <tr>
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    <td>BBa_K1819000</td>
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    <td>Linker-GFP</td>
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    <td>Reporter part</td>
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    <td>Is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site</td>
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    <td>750</td>
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  </tr>
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  <tr>
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    <td>BBa_K1819002</td>
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    <td>TAT signal</td>
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    <td>Signalling part</td>
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    <td>Twin-arginine translocation signal</td>
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    <td>147</td>
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  </tr>
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  <tr>
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    <td>BBa_K1819004</td>
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    <td>GFP_I13507</td>
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    <td>Composite part</td>
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    <td>RFP regulation through promoter tetR (part R0040), dependent of tetracycline</td>
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    <td>2499</td>
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  </tr>
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 +
  <tr>
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    <td>BBa_K1819005</td>
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    <td>J23101 + linkerGFPTerm</td>
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    <td>Characterization circuit</td>
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    <td>Constitutive promotor from Anderson library (BBa_J23101) assembled to green fluorescent protein attached to linker</td>
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    <td>930</td>
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  </tr>
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  <tr>
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    <td>BBa_K1819006</td>
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    <td>J23106 + linkerGFPTerm</td>
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    <td>Characterization circuit</td>
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    <td>Constitutive promoter from Anderson library (BBa_J23106) assembled to green fluorescent protein attached to linker</td>
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    <td>930</td>
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  </tr>
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 +
  <tr>
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    <td>BBa_K1819007</td>
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    <td>J23117 + linkerGFPTerm</td>
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    <td>Characterization circuit</td>
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    <td>Constitutive promotor from Anderson library (BBa_J23117) assembled to green fluorescent protein attached to linker</td>
 +
    <td>930</td>
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  </tr>
 +
 +
  <tr>
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    <td>BBa_K1819008</td>
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    <td>Pl_I13507</td>
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    <td>Construction promoter test</td>
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    <td>Promoter test circuit to analyze the kill switch efficiency</td>
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    <td>2725</td>
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  </tr>
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 +
  <tr>
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    <td>BBa_K1819009</td>
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    <td>pVeg + GFP_Term</td>
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    <td>Characterization circuit</td>
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    <td>This device express GFP under a strong constitutive Bacillus promoter control (pVeg) (BBa_K823003)</td>
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    <td>1123</td>
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 +
  </tr>
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 +
  <tr>
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    <td>BBa_S05291</td>
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    <td>RBS-OmpA</td>
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    <td>Construction intermediate</td>
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    <td>It is composed of a RBS preceding an outer membrane protein A (OmpA), that displays proteins on cell surface.</td>
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    <td>484</td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td>BBa_S05307</td>
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    <td>GFP_P0440</td>
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    <td>Construction intermediate</td>
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    <td>This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR</td>
 +
    <td>1568</td>
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  </tr>
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 +
  <tr>
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    <td>BBa_S05308</td>
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    <td>Linker-GFP_Term</td>
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    <td>Construction intermediate</td>
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    <td>It is an intermediate construction of the export test circuit fused to a terminal region</td>
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    <td>887</td>
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  </tr>
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 +
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</table>
  
 
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Revision as of 18:11, 17 September 2015

List of Parts

Registry Code Name Role Description Length (bp)
BBa_K1819003 RoxA Protein coding Rubber oxygenase A (Rox A) from Xanthomonas sp. strain 35Y- catalyzes oxidative C-C cleavage of poly(cis-1,4-isoprene) 1954 (bp)
BBa_K1819001 Lcp Role Latex clearing protein (Lcp) from Streptomyces sp. strain K30 - catalyzes rubber cleavage at the cis double bonds 1138
BBa_K1819000 Linker-GFP Reporter part Is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site 750
BBa_K1819002 TAT signal Signalling part Twin-arginine translocation signal 147
BBa_K1819004 GFP_I13507 Composite part RFP regulation through promoter tetR (part R0040), dependent of tetracycline 2499
BBa_K1819005 J23101 + linkerGFPTerm Characterization circuit Constitutive promotor from Anderson library (BBa_J23101) assembled to green fluorescent protein attached to linker 930
BBa_K1819006 J23106 + linkerGFPTerm Characterization circuit Constitutive promoter from Anderson library (BBa_J23106) assembled to green fluorescent protein attached to linker 930
BBa_K1819007 J23117 + linkerGFPTerm Characterization circuit Constitutive promotor from Anderson library (BBa_J23117) assembled to green fluorescent protein attached to linker 930
BBa_K1819008 Pl_I13507 Construction promoter test Promoter test circuit to analyze the kill switch efficiency 2725
BBa_K1819009 pVeg + GFP_Term Characterization circuit This device express GFP under a strong constitutive Bacillus promoter control (pVeg) (BBa_K823003) 1123
BBa_S05291 RBS-OmpA Construction intermediate It is composed of a RBS preceding an outer membrane protein A (OmpA), that displays proteins on cell surface. 484
BBa_S05307 GFP_P0440 Construction intermediate This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR 1568
BBa_S05308 Linker-GFP_Term Construction intermediate It is an intermediate construction of the export test circuit fused to a terminal region 887