Difference between revisions of "Team:UC San Diego/Notebook"
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| − | <p> | + | <p>Started interlab study.</p> |
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| − | <p>+Sent our genes to SGI and ordered lab supplies. </p> | + | <p>+Sent our genes to SGI and ordered lab supplies. Almost ready to go!</p> |
<span class="cd-date">WEEK 4</span> | <span class="cd-date">WEEK 4</span> | ||
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Revision as of 04:05, 18 September 2015
TIMELINE
September 24-28
Giant Jamboree!
Computational
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WEEK 14Wet Lab
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WEEK 14Title of section 2
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Section 13
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WEEK 13Wet Lab
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WEEK 13
Section 13
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WEEK 12week ##
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WEEK 12Section 13
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WEEK 11Section 13
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WEEK 11week ##
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WEEK 11Section 13
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WEEK 10Section 13
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WEEK 10week 10
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WEEK 10week 9
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WEEK 9/span>week 9
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WEEK 9week 9
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WEEK 9
Section 13
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WEEK 8week 8
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WEEK 8Section 13
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WEEK 7Section 13
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WEEK 7week 7
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WEEK 7Section 13
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WEEK 6Section 13
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WEEK 6Wet Lab
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WEEK 6Section 13
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WEEK 5Section 13
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WEEK 5Wet Lab
Started interlab study.
WEEK 5Section 13
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WEEK 4Section 13
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WEEK 4Wet Lab
+Sent our genes to SGI and ordered lab supplies. Almost ready to go!
WEEK 4Section 13
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WEEK 3Section 13
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WEEK 3Wet Lab
Added frp gene sequence from Vibrio Harveyi to our plasmid to stabilize the production of luciferase.
+Optimized our plasmid sequences.
Website
More website ideas.
WEEK 2Computational
Read papers on drive
Read about metabolic control analysis
Compiled notes on bioluminescent system
Literature research:understanding bioluminescent mechanism
> aldehyde synthesis
> FMNH2 production
> light production
Familiarized with COBRA module
Primer to Genome-Scale Modeled and COBRA Tutorial (Jahir)
Continued literature search for key mechanisms regarding our auto-induced bioluminescent reaction in a yeast model
>auto-induced therefore no coupling between cells (removed LuxI and LuxR genes)
Wet Lab
+Created a preliminary design for the plasmids using ApE.
+Improved them over the week by adding tags, removed illegal restriction sites, and changing repetitive sequences.
Website
Discussed and designed website prototypes.
WEEK 1Computational
Developed Primer to MATLAB Programming
> lecture material from MIT course and UCSD course
> reading material from UCSD course
> study and practice material MIT course and UCSD course
Wet Lab
+ Found genes coding for fatty acid reductase complex that have been validated in an in vitro synthesis paper.
+ Planned how to assemble our plasmids and determined nucleotide sequences for lux A-E of Photobacterium Phosphoreum.
+ Compared the amino acid sequences to those of other organisms on BLAST to check for significant discrepancies in our sequence.