The collection of parts described herein forms a modular platform to determine which of the enzymes of the fatty acid reductase complex is rate limiting. Each permutation of the complex’s genes is compatible for coexpression with the luxAB construct. This methodology of stoichiometric expression can also be used to determine rate-limiting steps in other metabolic systems.
LuxABfrp - Coding - Engineered luxAB with Vibrio Harveyi frp; 2A linker present between each protein for stoichiometric expression.
LuxCDEC - Coding - Photobacterium Phosphoreum fatty acid reductase complex with overexpressed reductase; 2A linker present between each protein for stoichiometric expression.
LuxCDED - Coding - Photobacterium Phosphoreum fatty acid reductase complex with overexpressed transferase; 2A linker present between each protein for stoichiometric expression.
LuxCDEE - Coding - Photobacterium Phosphoreum fatty acid reductase complex with overexpressed synthetase; 2A linker present between each protein for stoichiometric expression.
LuxDEC - Coding - Photobacterium Phosphoreum fatty acid reductase complex with even stoichiometry for LuxCDE. 2A linker present between each protein for stoichiometric expression.